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酚氧化酶活性与裂褶菌子实体形成

Phenoloxidase activity and fruiting body formation Schizophyllum commune.

作者信息

Leonard T J

出版信息

J Bacteriol. 1971 Apr;106(1):162-7. doi: 10.1128/jb.106.1.162-167.1971.

Abstract

Studies of induced haploid fruiting in the Basidiomycete Schizophyllum commune suggested that polyphenoloxidase enzymes may participate in the formation of fruiting bodies. Reported inhibitors of phenoloxidases were found to affect both the activity of these enzymes and the fruiting response. Fertile mycelia were found to give a positive Bavendamm reaction in situ, and cell-free extracts from fertile mycelia exhibited polyphenoloxidase activity when studied spectrophotometrically. Sterile mycelia, on the other hand, were devoid of these activities. Various kinds of mutant homo- and dikaryotic mycelia were studied, and the results suggested a correlation between phenoloxidase activity and fruiting body formation in S. commune.

摘要

对担子菌裂褶菌中诱导单倍体结实的研究表明,多酚氧化酶可能参与子实体的形成。已报道的酚氧化酶抑制剂被发现会影响这些酶的活性以及结实反应。发现可育菌丝体在原位呈现阳性的巴文德姆反应,并且当用分光光度法研究时,可育菌丝体的无细胞提取物表现出多酚氧化酶活性。另一方面,不育菌丝体则没有这些活性。对各种突变的同核和双核菌丝体进行了研究,结果表明裂褶菌中酚氧化酶活性与子实体形成之间存在相关性。

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