人源二聚体IgA超微结构的进一步研究。
Further studies on the ultrastructure of dimeric IgA of human origin.
作者信息
Bloth B, Svehag S E
出版信息
J Exp Med. 1971 May 1;133(5):1035-42. doi: 10.1084/jem.133.5.1035.
Abstract
Human colostral IgA and myeloma dimer IgA were purified and examined in the electron microscope using a modified technique of negative staining. Both types of preparation contained double Y-shaped structures of the dimensions: Fab region, 35 x 70 A, and the sum of the two Fc regions, 40 x 140-155 A. Colostral IgA as well as myeloma dimer IgA molecules showed a tendency of bending at the point where the Fc regions joined. Secretory component bound to dimer IgA produced no visible alteration of the molecule. Mild reduction and alkylation of colostral IgA yielded single Y-shaped 7S monomers with the dimensions, Fab, 35 x 70 A, and Fc, 40 x 65-70 A.
摘要
人初乳 IgA 和骨髓瘤二聚体 IgA 经纯化后，采用改良的负染色技术在电子显微镜下进行检测。两种制剂均含有尺寸如下的双 Y 形结构：Fab 区，35×70 Å；两个 Fc 区之和，40×140 - 155 Å。初乳 IgA 以及骨髓瘤二聚体 IgA 分子在 Fc 区连接点处呈现弯曲趋势。与二聚体 IgA 结合的分泌成分未使分子产生可见变化。初乳 IgA 的轻度还原和烷基化产生了尺寸为 Fab 35×70 Å、Fc 40×65 - 70 Å 的单 Y 形 7S 单体。
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