十二烷基硫酸钠在琼脂扩散和免疫电泳中引起血清蛋白的非特异性沉淀。

Palmer E L, Martin M L, Hierholzer J C, Ziegler D W

Appl Microbiol. 1971 May;21(5):903-6. doi: 10.1128/am.21.5.903-906.1971.

The anionic detergent sodium lauryl sulfate (SLS), in a final concentration of 0.1% and greater, reacted with whole serum in agar diffusion and immunoelectrophoresis to form artifactual precipitin lines. These lines occurred when either Ionagar or agarose was used as the supporting gel and were not affected by the presence of urea and 2-mercaptoethanol. Analytic chemical tests confirmed that the precipitating agent is SLS, and staining techniques showed that the detergent precipitates both protein and lipoprotein components of whole serum. Multiple artifactual precipitin lines occurred with a wide variety of animal sera, and a single line formed with human 7S immunoglobulin. Hence, in agar diffusion studies in which SLS is present in the test system, these artifactual lines may be easily misinterpreted as true antigen-antibody precipitin reactions.

阴离子去污剂十二烷基硫酸钠（SLS），终浓度达到0.1%及以上时，在琼脂扩散和免疫电泳中会与全血清发生反应，形成人为的沉淀线。当使用离子琼脂或琼脂糖作为支持凝胶时会出现这些沉淀线，且不受尿素和2-巯基乙醇存在的影响。分析化学测试证实沉淀剂是SLS，染色技术表明该去污剂会沉淀全血清中的蛋白质和脂蛋白成分。多种动物血清会出现多条人为沉淀线，而人7S免疫球蛋白会形成一条沉淀线。因此，在测试系统中存在SLS的琼脂扩散研究中，这些人为沉淀线可能很容易被误判为真正的抗原-抗体沉淀反应。