Miller L H, Usami S, Chien S
J Clin Invest. 1971 Jul;50(7):1451-5. doi: 10.1172/JCI106629.
Red cells parasitized by Plasmodium knowlesi concentrate within the microcirculation of many organs including cerebral capillaries in rhesus monkeys. The possibility that P. knowlesi could alter the rheologic properties of red cells so that they are trapped within capillaries was investigated in the present study. The viscosity of P. knowlesi-infected red cells suspended in Ringer's solution was increased at all shear rates at hematocrits above 30%. At moderate parasitemia the resistance to flow through 5 mu polycarbonate sieves was increased; at high parasitemia the pores were obstructed. Mature trophozoites caused more obstruction than young trophozoites (rings) at any given level of parasitemia. The reduction of deformability of red cells infected by schizonts of P. knowlesi was further demonstrated by their exclusion from rouleaux in a plasma suspension. Therefore, the red cells infected by P. knowlesi become less deformable, and this reduction in red cell deformability may explain the obstruction of cerebral capillaries.
感染诺氏疟原虫的红细胞会在包括恒河猴脑毛细血管在内的许多器官的微循环中聚集。本研究调查了诺氏疟原虫是否会改变红细胞的流变学特性，使其被困在毛细血管中。在血细胞比容高于30%时，悬浮在林格氏溶液中的感染诺氏疟原虫的红细胞在所有剪切速率下粘度均增加。在中等虫血症时，通过5微米聚碳酸酯筛网的流动阻力增加；在高虫血症时，孔隙被阻塞。在任何给定的虫血症水平下，成熟滋养体比年轻滋养体（环状体）造成的阻塞更多。感染诺氏疟原虫裂殖体的红细胞从血浆悬浮液中的缗钱状聚集物中被排除，这进一步证明了其变形性的降低。因此，感染诺氏疟原虫的红细胞变形性降低，而这种红细胞变形性的降低可能解释了脑毛细血管的阻塞。
