Aschauer H, Zaidi Z H, Braunitzer G
Hoppe Seylers Z Physiol Chem. 1979 Oct;360(10):1513-6.
The complete amino acid sequence of 147 residues was determined automatically for a major dimeric component (CTT VI) of the insect larva Chironomus thummi thummi (Diptera). The molweight was found to be 32411. All tryptic, maleylated tryptic and cyanogen bromide peptides were isolated. The handling of some large fragments was facilitated by maleylation and subsequent ion exchange chromatography. Some details of the primary structure are discussed. The alignment of the amino acid sequence with that of human alpha-chains shows only 29 identical positions.
已自动测定了摇蚊（双翅目）幼虫主要二聚体成分（CTT VI）147个残基的完整氨基酸序列。发现其分子量为32411。分离出了所有胰蛋白酶、马来酰化胰蛋白酶和溴化氰肽段。马来酰化及随后的离子交换色谱法有助于处理一些大片段。讨论了一级结构的一些细节。该氨基酸序列与人α链氨基酸序列的比对仅显示出29个相同位置。
