牛红细胞膜抗原的分离：与唾液蛋白相关的抗原。
The isolation of ox red cell membrane antigens: antigens associated with sialoprotein.
作者信息
Spooner R L, Maddy A H
出版信息
Immunology. 1971 Nov;21(5):809-16.
Abstract
Ox red cell antigens F, V, ε, J, L, R′, S′ and T′ have been recovered in soluble membrane protein obtained by a butanol extraction method. F, V and ε have been studied in more detail and are found in the sialic acid rich fraction of the membrane protein. Isoimmunization with protein containing F and V antigens produced excellent specific antisera for these antigens. Antigens lost in butanol extraction were not destroyed by sonication, and it has not been possible to regenerate activity for these lost antigens.
摘要
牛红细胞抗原F、V、ε、J、L、R′、S′和T′已在通过丁醇提取法获得的可溶性膜蛋白中得以回收。对F、V和ε进行了更详细的研究，发现它们存在于膜蛋白富含唾液酸的部分。用含有F和V抗原的蛋白质进行同种免疫，产生了针对这些抗原的优质特异性抗血清。在丁醇提取过程中丢失的抗原不会因超声处理而被破坏，而且无法使这些丢失的抗原恢复活性。
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