伴刀豆球蛋白A处理的小鼠淋巴细胞的细胞电泳研究：细胞表面可能存在两种类型的受体。
Cell electrophoretic studies on mouse lymphocytes treated with concanavalin A: possible existence of two types of receptors on cell surface.
作者信息
Sainis K B, Bhisey A N, Sundaram K, Phondke G P
出版信息
Immunology. 1979 Jul;37(3):555-61.
Concanavalin A (Con A) induced biphasic changes in the electrophoretic mobility (EPM) of splenic lymphocytes of AKR mice. At low concentrations of Con A (2-12.5 mug/ml) there was a significant increase in there was a significant increase in the EPM which could be attributed to the redistribution and endocytosis of Con A receptors. At higher concentrations (greater than or equal to 15.0 mug/ml) a reduction in EPM below that of untreated cells was possibly due to the binding of excess Con A after redistribution. The biphasic profile of EPM was observed only in respect of Con A-treated T lymphocytes. The EPM of B lymphocytes, despite binding of Con A, remained unaltered at all concentrations of Con A. On the basis of these data, the existence of two types of Con A receptors on the cell surface is proposed and their status vis-a-vis the redistribution is discussed.
伴刀豆球蛋白A（Con A）可诱导AKR小鼠脾淋巴细胞的电泳迁移率（EPM）发生双相变化。在低浓度的Con A（2 - 12.5微克/毫升）时，EPM显著增加，这可归因于Con A受体的重新分布和内吞作用。在较高浓度（大于或等于15.0微克/毫升）时，EPM低于未处理细胞，这可能是由于重新分布后过量Con A的结合所致。EPM的双相特征仅在Con A处理的T淋巴细胞中观察到。B淋巴细胞的EPM，尽管Con A与之结合，但在所有Con A浓度下均保持不变。基于这些数据，提出了细胞表面存在两种类型的Con A受体，并讨论了它们相对于重新分布的状态。
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