Wu T C, Park J T
J Bacteriol. 1971 Nov;108(2):874-84. doi: 10.1128/jb.108.2.874-884.1971.
A mutant of Staphylococcus aureus H was isolated by virtue of its inability to agglutinate with antibodies against teichoic acid of S. aureus. Immunological studies revealed that the mutant, S. aureus T, possessed a new surface antigen in addition to having the antigenic determinant of the wild-type strain, the ribitol teichoic acid. The presence of this additional surface component rendered strain T resistant to staphylococcal typing phages, presumably by masking the phage-receptor sites. The polymer was separated from teichoic acid by chromatography on diethylaminoethyl cellulose and was shown to be composed of two amino sugars, N-acetyl-d-fucosamine and N-acetyl-d-mannosamin uronic acid.
通过无法与抗金黄色葡萄球菌磷壁酸的抗体发生凝集反应，分离出了金黄色葡萄球菌H的一个突变体。免疫学研究表明，该突变体金黄色葡萄球菌T除了具有野生型菌株的抗原决定簇——核糖醇磷壁酸外，还拥有一种新的表面抗原。这种额外表面成分的存在使T菌株对葡萄球菌分型噬菌体具有抗性，可能是通过掩盖噬菌体受体位点实现的。通过在二乙氨基乙基纤维素上进行色谱分离，将该聚合物与磷壁酸分离，并显示其由两种氨基糖组成，即N-乙酰基-D-岩藻糖胺和N-乙酰基-D-甘露糖胺糖醛酸。
