铜绿假单胞菌中扁桃酸途径的调控
Regulation of the mandelate pathway in Pseudomonas aeruginosa.
作者信息
Rosenberg S L
出版信息
J Bacteriol. 1971 Dec;108(3):1257-69. doi: 10.1128/jb.108.3.1257-1269.1971.
The pathway of mandelate metabolism in Pseudomonas aeruginosa is composed of the following steps: l(+)-mandelate --> benzoylformate --> benzaldehyde --> benzoate. These three steps are unique to mandelate oxidation; the benzoate formed is further metabolized via the beta-ketoadipate pathway. The first enzyme, l(+)-mandelate dehydrogenase, is induced by its substrate. The second and third enzymes, benzoylformate decarboxylase and benzaldehyde dehydrogenase, are both induced by benzoylformate. The same benzaldehyde dehydrogenase, or one very similar to it, is also induced by beta-ketoadipate, an intermediate in the subsequent metabolism of benzoate. This dehydrogenase may also be induced by adipate or a metabolite of adipate. These conclusions have been drawn from the physiological and genetic properties of wild-type P. aeruginosa strains and from the study of mutants lacking the second and third enzyme activities.
铜绿假单胞菌中扁桃酸代谢途径由以下步骤组成
l(+)-扁桃酸→苯甲酰甲酸→苯甲醛→苯甲酸。这三个步骤是扁桃酸氧化所特有的；生成的苯甲酸通过β-酮己二酸途径进一步代谢。第一种酶，l(+)-扁桃酸脱氢酶，由其底物诱导产生。第二种和第三种酶，苯甲酰甲酸脱羧酶和苯甲醛脱氢酶，均由苯甲酰甲酸诱导产生。同一种苯甲醛脱氢酶，或与其非常相似的一种酶，也由β-酮己二酸诱导产生，β-酮己二酸是苯甲酸后续代谢过程中的一种中间产物。这种脱氢酶也可能由己二酸或己二酸的一种代谢产物诱导产生。这些结论是从野生型铜绿假单胞菌菌株的生理和遗传特性以及对缺乏第二种和第三种酶活性的突变体的研究中得出的。
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