Ono K
Histochemistry. 1979 Aug;62(2):113-24. doi: 10.1007/BF00493313.
Ultrastructural localization of acid phosphatase activity was investigated in ultrathin (0.05 micron) and semithin (0.5 and 0.75 micron) sections of the small intestinal epithelial cells of adult rats. The results showed that the enzyme activity was localized on the membrane of microvilli, lateral cell membranes, lysosomes, the Golgi complex, and the GERL of Novikoff (a part of the smooth-surfaced endoplasmic reticulum located in close proximity to the inner Golgi saccules) of duodenal absorptive cells. The lysosomes contained within the duodenal and jejunal absorptive cells appeared to be mainly heterolysosomes rather than autolysosomes. The enzyme activity of absorptive cells was lower in the jejunum than in the duodenum, and was barely detectable except in the GERL and lysosomes of the ileum. The average numbers of lysosomes having a diameter of 0.2 approximately 1.0 microns, per cell profile in sections of 214 duodenal, 226 jejunal and 318 ileal epithelial cells were 8.9 +/- 0.189, 6.4 +/- 0.155 and 3.5 +/- 0.027 (mean +/- SE), respectively. From these results, it was assumed that both the Golgi apparatus and GERL produce some lysosomes in the duodenal and jejunal absorptive cells, but only GERL does so in the ileum. It was considered also that because of an unexpectedly high number of lysosomes containes within the epithelial absorptive cells of the proximal intestine of adult rats, these cells may possess the strong heterophagic, as well as absorptive capacity.
对成年大鼠小肠上皮细胞超薄（0.05微米）和半薄（0.5和0.75微米）切片中的酸性磷酸酶活性进行了超微结构定位研究。结果表明，该酶活性定位于十二指肠吸收细胞的微绒毛膜、侧细胞膜、溶酶体、高尔基体以及诺维科夫（Novikoff）的GERL（位于靠近高尔基体内囊泡的光滑内质网的一部分）上。十二指肠和空肠吸收细胞内的溶酶体似乎主要是异噬性溶酶体而非自噬性溶酶体。空肠吸收细胞的酶活性低于十二指肠，且除了回肠的GERL和溶酶体外几乎检测不到。在214个十二指肠、226个空肠和318个回肠上皮细胞切片中，每个细胞轮廓中直径约为0.2至1.0微米的溶酶体平均数量分别为8.9±0.189、6.4±0.155和3.5±0.027（平均值±标准误）。根据这些结果推测，高尔基体和GERL在十二指肠和空肠吸收细胞中都产生一些溶酶体，但在回肠中只有GERL产生溶酶体。还认为，由于成年大鼠近端肠道上皮吸收细胞内溶酶体数量出乎意料地多，这些细胞可能具有很强的异噬能力以及吸收能力。
