交感神经节去污剂提取的神经生长因子受体的物理性质

Physical properties of the detergent-extracted nerve growth factor receptor of sympathetic ganglia.

作者信息

Costrini N V, Kogan M, Kukreja K, Bradshaw R A

出版信息

J Biol Chem. 1979 Nov 25;254(22):11242-6.

Abstract

The nerve growth factor (NGF) receptor from microsomes of adult rabbit superior cervical ganglia has been solubilized with Triton X-100 and sodium deoxycholate. The physical properties of the detergent-extracted NGF receptor were assessed by Sepharose 6B chromatography and sucrose density gradient ultracentrifugation studies in H2O and D2O. The predominant form of the NGF receptor has a Stokes radius of 71 A, a partial specific volume of 0.74 ml/g, a sedimentation coefficient of 4.3 S, and a frictional ratio of 1.8. From these parameters, it can be calculated that the NGF receptor in Triton X-100 is a minimally hydrophobic, highly asymmetric, intrinsic membrane protein with a molecular weight of approximately 135,000. A form of the receptor with a sedimentation coefficient of 10.4 S was occasionally seen which appears to represent an aggregated form of the 4.3 S moiety.

摘要

成年兔颈上神经节微粒体中的神经生长因子（NGF）受体已用 Triton X - 100 和脱氧胆酸钠进行了增溶处理。通过在 H₂O 和 D₂O 中进行琼脂糖 6B 层析和蔗糖密度梯度超速离心研究，评估了去污剂提取的 NGF 受体的物理性质。NGF 受体的主要形式具有 71 Å 的斯托克斯半径、0.74 ml/g 的偏比容、4.3 S 的沉降系数和 1.8 的摩擦比。根据这些参数可以计算出，Triton X - 100 中的 NGF 受体是一种疏水性最低、高度不对称的内在膜蛋白，分子量约为 135,000。偶尔会出现一种沉降系数为 10.4 S 的受体形式，它似乎代表了 4.3 S 部分的聚集形式。