阿德利企鹅（Pygoscelis adeliae）的血浆蛋白和免疫球蛋白
Plasma proteins and immunoglobulins in penguins (Pygoscelis adeliae).
作者信息
Castrelos O D, Mercado M R, Abatángelo C, Margni R A
出版信息
Biochim Biophys Acta. 1975 Jul 21;400(1):137-42. doi: 10.1016/0005-2795(75)90134-8.
Total and fractionated proteins were evaluated in the serum of normal penguins. Four proteins with antibody activity, determined by passive haemagglutination, were isolated by Sephadex G-200 filtration and DEAE-cellulose chromatography, from the serum of penguins (Pygoscelis adeliae) inoculated with dinitrophenylated human gamma-globulin. Immunoelectrophoresis and immunodiffusion showed that all these proteins exhibited both their own and shared antigenic determinants. On account of their electrophoretic mobility, elution conditions and characteristics of the precipitation arcs, they have been tentatively denominated IgG2 (gamma2), IgG1 (gamma1), IgM (gammam) and IgX (probably IgA).
对正常企鹅血清中的总蛋白和分级蛋白进行了评估。通过葡聚糖凝胶G - 200过滤和二乙氨基乙基纤维素色谱法，从接种二硝基苯基化人γ球蛋白的阿德利企鹅（Pygoscelis adeliae）血清中分离出四种具有抗体活性的蛋白，其抗体活性通过被动血凝反应测定。免疫电泳和免疫扩散表明，所有这些蛋白都表现出自身的和共有的抗原决定簇。根据它们的电泳迁移率、洗脱条件和沉淀弧的特征，它们被初步命名为IgG2（γ2）、IgG1（γ1）、IgM（γm）和IgX（可能是IgA）。
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