Blume P, Greenberg L J
Clin Chem. 1975 Aug;21(9):1234-7.
When a suspension of particle is irradiated with a collimated beam of monochromatic polarized light of a wavelength close to the particle size and the intensity of the scattered light is measured as a function of angle, the scatter intensity is characterized by a series of relative maxima and minima. The nature of the signal depends on several significant variables that are characteristic of the particles. We have constructed a differential light scatter photometer and have applied the technique to analysis of rheumatoid factor by using latex particles coated with fraction II gamma-globulin. The results suggest that such a photometer may have potential applications in antigen-antibody assays based on the use of sensitized particles.
当用一束波长接近颗粒大小的准直单色偏振光照射颗粒悬浮液，并测量散射光强度随角度的变化时，散射强度具有一系列相对的最大值和最小值。信号的性质取决于颗粒的几个重要特征变量。我们构建了一台差分光散射光度计，并将该技术应用于通过使用包被有II型γ球蛋白的乳胶颗粒来分析类风湿因子。结果表明，这种光度计在基于使用致敏颗粒的抗原-抗体测定中可能具有潜在应用。
