Barratt M E
Immunology. 1972 Apr;22(4):615-23.
Saline extracts of adult spp. were fractionated by both Sephadex gel filtration and anion exchange chromatography, and the fractions tested for allergenicity both by wheal and erythema tests in infected pigs, and by P-K tests in passively sensitized pigs. It is suggested that cross reactions, so commonly found when using nematode antigens in wheal and erythema reactions, can be eliminated by suitable dilution of the allergen. It was shown that the allergenic components had a molecular weight range of 5000 to 50,000, and were eluted from DEAE cellulose with a series of buffers of differing molarity.
对成年某种寄生虫的盐提取物进行了葡聚糖凝胶过滤和阴离子交换色谱分离，并通过在感染猪身上进行风团和红斑试验以及在被动致敏猪身上进行P-K试验来检测各馏分的致敏性。研究表明，在风团和红斑反应中使用线虫抗原时常见的交叉反应，可以通过适当稀释变应原加以消除。结果显示，致敏成分的分子量范围为5000至50000，并且用一系列不同摩尔浓度的缓冲液从DEAE纤维素上洗脱下来。
