McAninch J R, Patterson R
Immunology. 1970 Jan;18(1):91-8.
A heterogeneous antibody response was observed in rabbits immunized with crude antigens or pooled active fractions prepared by gelfiltration chromatography. The development of haemagglutinating antibodies, precipitating antibodies and reagin-like (homocytotropic) antibodies was observed. The reagin-like antibodies were shown to be heat-labile, 2-mercaptoethanol sensitive and clearly different from 7S IgG antibodies when localized by Sephadex G-200 gel-filtration chromatography.
在用粗抗原或通过凝胶过滤色谱法制备的混合活性组分免疫的兔子中观察到异质性抗体反应。观察到血凝抗体、沉淀抗体和反应素样(亲同种细胞性)抗体的产生。反应素样抗体表现为热不稳定、对2-巯基乙醇敏感,并且当通过Sephadex G-200凝胶过滤色谱法定位时,与7S IgG抗体明显不同。