补体在抗淋巴细胞血清的玫瑰花结抑制和淋巴细胞毒性中的作用机制。
Mechanism of complement action in rosette inhibition and lymphocytotoxicity of antilymphocyte serum.
作者信息
Bach J F, Gigli I, Dardenne M, Dormont J
出版信息
Immunology. 1972 Apr;22(4):625-35.
Rosette inhibition (RI) was obtained in the absence of complement, but was generally much enhanced by the presence of complement. RI titres measured in the presence of complement correlated well with immunosuppressive potency. In the absence of complement, RI was due to anti-immunoglobulin antibodies contained in ALS and did not correlate with immunosuppressive potency. Complement enhancement of RI required only C[unk]1 and C4 and C[unk]1 used alone had a significant enhancing potency. The effect of complement developed within 5 minutes on ALS-treated lymphocytes. Rosette inhibition was obtained with concentrations of ALS and complement much lower than those necessary for cytotoxicity. With crude rabbit ALS a competition was observed between inactivated rabbit complement and guinea-pig complement. Lymphocytotoxicity was obtained after 90 minutes' incubation of lymphocytes with ALS in the presence of complement. Rabbit ALS was cytotoxic in the presence of rabbit or guinea-pig complement. Horse ALS fixed rabbit but not guinea-pig complement. The whole sequence of the nine components was necessary for the cytotoxic reaction.
在没有补体的情况下可获得玫瑰花结抑制（RI），但补体的存在通常会使其大大增强。在有补体存在的情况下测得的RI效价与免疫抑制效力密切相关。在没有补体的情况下，RI是由于抗淋巴细胞血清（ALS）中所含的抗免疫球蛋白抗体所致，且与免疫抑制效力无关。补体对RI的增强作用仅需要C[unk]1和C4，单独使用C[unk]1就具有显著的增强效力。补体的作用在5分钟内就出现在经ALS处理的淋巴细胞上。获得玫瑰花结抑制所需的ALS和补体浓度远低于细胞毒性所需的浓度。使用粗制兔ALS时，观察到灭活的兔补体和豚鼠补体之间存在竞争。在有补体存在的情况下，淋巴细胞与ALS孵育90分钟后会产生淋巴细胞毒性。兔ALS在有兔或豚鼠补体存在时具有细胞毒性。马ALS能结合兔补体，但不能结合豚鼠补体。细胞毒性反应需要九种补体成分的完整序列。
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