Montazemi K, Lines J G
J Clin Pathol. 1972 Feb;25(2):141-8. doi: 10.1136/jcp.25.2.141.
Measurement of serum hydroxybutyrate dehydrogenase (HBD) activity for suspected myocardial infarction will nowadays usually be carried out with a commercially available test kit. Four such kits have been compared and evaluated: British Drug Houses HBDH set, Boehringer Corporation LDH-1-Iso-enzyme ;alpha-HBDH' kit, Calbiochem alpha-HBDH Statpack, and Eskalab alpha-HBDH reagent tablets. Of the 100 sera examined, 60 were from patients believed to have suffered a recent myocardial infarction, and 40 samples were from patients aged between 45 and 65 years who had no known hepatic, renal, or cardiac pathology, these being used to determine the normal range for each kit. Under standardized conditions the activity found differed from kit to kit; the clinical value of the various assay systems in terms of their ability to discriminate between normal and pathological sera is assessed; and finally the cost and the convenience of use of each kit procedure are discussed. Theoretical and experimental evidence is provided in support of an assay temperature of 30 degrees C, and a plea is made for international agreement on enzyme assay conditions.
如今，对于疑似心肌梗死患者，血清羟丁酸脱氢酶（HBD）活性的测定通常使用市售检测试剂盒来进行。已对四种此类试剂盒进行了比较和评估：英国药品公司的HBDH套装、勃林格殷格翰公司的LDH - 1同工酶α - HBDH试剂盒、Calbiochem公司的α - HBDH Statpack以及Eskalab公司的α - HBDH试剂片。在检测的100份血清中，60份来自被认为近期发生心肌梗死的患者，40份样本来自年龄在45至65岁之间、无已知肝脏、肾脏或心脏病变的患者，这些样本用于确定每个试剂盒的正常范围。在标准化条件下，不同试剂盒测得的活性有所不同；评估了各种检测系统在区分正常和病理血清方面的临床价值；最后讨论了每个试剂盒操作的成本和使用便利性。提供了理论和实验证据来支持30摄氏度的测定温度，并呼吁就酶测定条件达成国际协议。
