Kurisaki E, Sato H, Nagamori H
J Toxicol Sci. 1979 May;4(2):99-104. doi: 10.2131/jts.4.99.
The effects of paraquat on human embryonic somatic cells were studied. Monolayer cultured cells, from several different organs, were exposed to various concentrations of paraquat. Then, the cells were stained with nigrosine and Sudan IV to demonstrate dead or damaged cells and fatty granules, respectively. The lowest paraquat concentration that caused morphological changes varied from 1 to 10 ppm, and the lowest concentration that caused fatty degeneration varied from 1 to 5 ppm. In both types of staining, the lowest degenerative concentration was lower as the culture generation was younger, and the degree of degeneration did not differ with the cell origin. The protein content per cell in paraquat-treated cells was not significantly decreased in comparison with control cells.
研究了百草枯对人胚胎体细胞的影响。将来自几个不同器官的单层培养细胞暴露于不同浓度的百草枯中。然后，分别用尼罗蓝和苏丹IV对细胞进行染色，以显示死亡或受损细胞以及脂肪颗粒。导致形态变化的最低百草枯浓度在1至10 ppm之间，导致脂肪变性的最低浓度在1至5 ppm之间。在两种染色类型中，随着培养代数越年轻，最低变性浓度越低，并且变性程度与细胞来源无关。与对照细胞相比，百草枯处理细胞中每细胞的蛋白质含量没有显著降低。
