链状库特氏菌产生脯氨酸的机制。
Mechanism of proline production by Kurthia catenaforma.
作者信息
Kato J, Fukushima H, Kisumi M, Chibata I
出版信息
Appl Microbiol. 1972 Apr;23(4):699-703. doi: 10.1128/am.23.4.699-703.1972.
The fate of aspartic acid used for proline fermentation by Kurthia catenaforma was traced by using aspartic acid-U-(14)C. The radioactivities of proline and glutamic acid increased with the disappearance of aspartic acid. After 40 hr, aspartic acid disappeared from the medium and radioactive alpha-ketoglutaric acid was detected. The radioactivity of proline reached 44% of aspartic acid radioactivity at 40 hr. The specific radioactivities of these amino acids and of alpha-ketoglutaric acid supported the notion that proline is produced mainly from aspartic acid via alpha-ketoglutaric acid and glutamic acid. Since the levels of glutamic acid dehydrogenases (EC 1.4.1.2 and EC 1.4.1.4) were low in this organism, it appears that the nitrogen atom of aspartic acid enters proline by the action of aspartate aminotransferase (EC 2.6.1.1). The mechanism of proline production is discussed on the basis of the role of aspartic acid in this fermentation.
利用天冬氨酸 -U-(14)C追踪了链状库尔希亚菌用于脯氨酸发酵的天冬氨酸的去向。随着天冬氨酸的消失，脯氨酸和谷氨酸的放射性增加。40小时后，天冬氨酸从培养基中消失，并检测到放射性α-酮戊二酸。40小时时，脯氨酸的放射性达到天冬氨酸放射性的44%。这些氨基酸和α-酮戊二酸的比放射性支持了脯氨酸主要通过α-酮戊二酸和谷氨酸从天冬氨酸产生的观点。由于该生物体中谷氨酸脱氢酶（EC 1.4.1.2和EC 1.4.1.4）的水平较低，天冬氨酸的氮原子似乎是通过天冬氨酸转氨酶（EC 2.6.1.1）的作用进入脯氨酸的。基于天冬氨酸在这种发酵中的作用，讨论了脯氨酸的产生机制。
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