Bondareff W, Narotzky R
Science. 1972 Jun 9;176(4039):1135-6. doi: 10.1126/science.176.4039.1135.
The extracellular space of the rat brain was visualized by electron roscopy in sections of cerebral cortex fixed by freeze-substitution. The volume tissue occupied by the extracellular space was estimated stereomnetrically, and found to decrease from 20.8 percent in 3-month-old rats to 9.6 percent in escent animals, 26 months of age. This decrease in extracellular space indicates age-associated change in the microenvironment of nerve cells.
