Baron D N, Buttery J E
J Clin Pathol. 1972 May;25(5):415-21. doi: 10.1136/jcp.25.5.415.
The electrophoretic separations of some human and pig liver enzymes on cellulose acetate and Cellogel were investigated, with reference to their joint occurrence in serum of patients undergoing treatment by extracorporeal pig liver perfusion. In every case it was possible to distinguish between the human and pig enzymes. Pig lactate dehydrogenase isoenzymes occupy a position slightly anodic to the corresponding human bands. The aspartate transaminase band of human is more anodic than that of pig, but their cathodic bands have the same mobility. Alanine transaminase of both human and pig liver extract is shown to exist as two bands each towards the anode. The faster moving human band is more anodic than the corresponding pig band, while the other human band is less anodic. Sorbitol dehydrogenase, alkaline phosphatase, and ornithine carbamoyltransferase all exist as one band each. Human sorbitol dehydrogenase is more cathodic than the pig enzyme, human alkaline phosphatase more anodic than the pig enzyme, while human ornithine carbamoyltransferase is less anodic than the pig enzyme.
研究了一些人和猪肝酶在醋酸纤维素和赛洛凝胶上的电泳分离情况,并参考了它们在接受体外猪肝灌注治疗的患者血清中的共同存在情况。在每种情况下,都能够区分人和猪的酶。猪乳酸脱氢酶同工酶的位置比相应的人酶带略偏阳极。人天冬氨酸转氨酶带比猪的更偏阳极,但它们的阴极带迁移率相同。人和猪肝提取物中的丙氨酸转氨酶均显示向阳极各存在两条带。迁移较快的人酶带比相应的猪酶带更偏阳极,而另一条人酶带则偏阳极程度较小。山梨醇脱氢酶、碱性磷酸酶和鸟氨酸氨甲酰转移酶均各以一条带存在。人山梨醇脱氢酶比猪酶更偏阴极,人碱性磷酸酶比猪酶更偏阳极,而人鸟氨酸氨甲酰转移酶比猪酶偏阳极程度较小。