Takada A, Urano T, Takada Y
Thromb Haemost. 1979 Oct 31;42(3):901-8.
Human plasma was mixed with Ca++ or thrombin, and urokinase (UK) or streptokinase (SK) and a chromogenic substrate (S-2251: H-D-Val-Leu-Lys-pNA) specific to plasmin. The hydrolysis of S-2251 was higher when clot was formed by the addition of Ca++ or thrombin than in the absence of clot. The hydrolysis of S-2251 by euglobulin in the presence of UK was also higher when clot was formed, thus, inhibitors may not be related to the better activation of plasminogen, in the presence of fibrin clot. It may be suggested that plasminogen was better activated by activators (UK and SK) in the clot than in its absence.
将人血浆与钙离子或凝血酶、尿激酶（UK）或链激酶（SK）以及纤溶酶特异性的发色底物（S - 2251：H - D - 缬氨酸 - 亮氨酸 - 赖氨酸 - 对硝基苯胺）混合。通过添加钙离子或凝血酶形成凝块时，S - 2251的水解程度高于未形成凝块时。在有UK存在的情况下，优球蛋白对S - 2251的水解在形成凝块时也更高，因此，在纤维蛋白凝块存在时，抑制剂可能与纤溶酶原的更好激活无关。可以推测，与不存在凝块时相比，凝块中的激活剂（UK和SK）能更好地激活纤溶酶原。
