羽扇豆和锯齿雀麦豆血红蛋白的分子特性
Molecular properties of lupin and serradella leghaemoglobins.
作者信息
Broughton W J, Dilworth M J, Godfrey C A
出版信息
Biochem J. 1972 Mar;127(1):309-14. doi: 10.1042/bj1270309.
Abstract
- Leghaemoglobins were extracted from the root nodules of lupin (Lupinus luteus L.) and serradella (Ornithopus sativus Brot.) plants and fractionated into different leghaemoglobin components on DEAE-cellulose-acetate columns. 2. The first two fractions eluted from columns loaded with either lupin or serradella leghaemoglobins were in the Fe(3+) oxidation state. 3. These components have protohaem IX as the prosthetic group and glycine as the N-terminal amino acid. 4. Other properties are: lupin component I, pI5.08, molecular weight 19000; lupin component II, pI5.13, molecular weight 20600; serradella component I, pI5.00, molecular weight 17500; serradella component II, pI5.05, molecular weight 19100. 5. Leghaemoglobins are thus heterogeneous with respect to size and charge.
摘要
- 从羽扇豆（羽扇豆属黄羽扇豆）和锯齿状三叶草（鸟足豆属普通鸟足豆）植物的根瘤中提取豆血红蛋白，并在二乙氨基乙基纤维素 - 醋酸纤维素柱上分离成不同的豆血红蛋白组分。2. 从装有羽扇豆或锯齿状三叶草豆血红蛋白的柱上洗脱的前两个组分处于Fe(3+)氧化态。3. 这些组分以原卟啉IX作为辅基，以甘氨酸作为N端氨基酸。4. 其他特性如下：羽扇豆组分I，pI5.08，分子量19000；羽扇豆组分II，pI5.13，分子量20600；锯齿状三叶草组分I，pI5.00，分子量17500；锯齿状三叶草组分II，pI5.05，分子量19100。5. 因此，豆血红蛋白在大小和电荷方面是异质的。
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