Agarwal M K, Parant M, Parant F
Br J Exp Pathol. 1972 Oct;53(5):485-91.
The nature of cellular factors in host response to endotoxin was determined by studying various phases of reactivity to endotoxin in splenectomized and sham operated mice. Spleen ablation by itself actually increased the resistance of animals to endotoxin lethality and the absence of spleen did not interfere with either the development or expression of tolerance to the lethal effects of the toxin. The clearance rates of carbon and of endotoxin were normal after splenectomy and increased in conjunction with RES activation associated with endotoxin tolerance, both in sham operated and the splenectomized animals. From these results, it is concluded that subtle and specific adaptations of hepatic RE elements and/or critical metabolic functions play a determining role in endotoxicosis. Furthermore, the presence of spleen does not seem to be required for opsonization, phagocytosis and removal of either nonspecific, inert colloidal particles such as carbon, or of biological materials such as endotoxin. Pertinence of these findings to splenectomized patients is indicated.
通过研究脾切除小鼠和假手术小鼠对内毒素反应的不同阶段，确定了宿主对内毒素反应中细胞因子的性质。脾脏切除本身实际上增加了动物对内毒素致死性的抵抗力，并且脾脏缺失并不干扰对毒素致死效应的耐受性的形成或表达。脾切除后碳和内毒素的清除率正常，并且在假手术动物和脾切除动物中，随着与内毒素耐受性相关的网状内皮系统（RES）激活而增加。从这些结果可以得出结论，肝脏RE元件和/或关键代谢功能的细微和特异性适应在内毒素血症中起决定性作用。此外，无论是调理作用、吞噬作用还是清除非特异性惰性胶体颗粒（如碳）或生物材料（如内毒素），似乎都不需要脾脏的存在。这些发现对脾切除患者的相关性也得到了说明。
