用1,5-二氟-2,4-二硝基苯和溴化乙锭所示精胺在细菌核糖体中的位置。
The location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide.
作者信息
Stevens L, Pascoe G
出版信息
Biochem J. 1972 Jun;128(2):279-89. doi: 10.1042/bj1280279.
Abstract
- When the binding of ethidium bromide to rRNA is measured both in the presence and in the absence of spermine, by spectrophotometric titrations, by gel filtration, or by the changes in fluorescence intensity, spermine competes with ethidium bromide for sites on the rRNA; under the conditions used in these experiments ethidium bromide is bound to the double-stranded regions of rRNA. 2. When an excess of ethidium bromide is added to ribosomes from Bacillus stearothermophilus approx. 80% of the endogenous spermine is displaced from the ribosomes. 3. [(14)C]Spermine is fixed to ribosomes by either formaldehyde or 1,5-difluoro-2,4-dinitrobenzene. Most of the [(14)C]spermine, fixed to ribosomes by 1,5-difluoro-2,4-dinitrobenzene, attaches to the ribosomal protein. 4. It is concluded that most of the endogenous spermine is bound to the double-stranded RNA in ribosomes, and that some of these double-stranded regions to which spermine is attached also have ribosomal proteins bound to them.
摘要
- 当通过分光光度滴定法、凝胶过滤法或荧光强度变化来测定溴化乙锭在有精胺和无精胺情况下与rRNA的结合时，精胺与溴化乙锭竞争rRNA上的位点；在这些实验所用的条件下，溴化乙锭与rRNA的双链区域结合。2. 当向嗜热脂肪芽孢杆菌的核糖体中加入过量的溴化乙锭时，约80%的内源性精胺会从核糖体上被置换下来。3. [¹⁴C]精胺可通过甲醛或1,5-二氟-2,4-二硝基苯固定到核糖体上。通过1,5-二氟-2,4-二硝基苯固定到核糖体上的大部分[¹⁴C]精胺会附着到核糖体蛋白上。4. 得出的结论是，大部分内源性精胺与核糖体中的双链RNA结合，并且一些与精胺结合的双链区域也结合有核糖体蛋白。
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