用1,5-二氟-2,4-二硝基苯和溴化乙锭所示精胺在细菌核糖体中的位置。
The location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide.
作者信息
Stevens L, Pascoe G
出版信息
Biochem J. 1972 Jun;128(2):279-89. doi: 10.1042/bj1280279.
Abstract
- When the binding of ethidium bromide to rRNA is measured both in the presence and in the absence of spermine, by spectrophotometric titrations, by gel filtration, or by the changes in fluorescence intensity, spermine competes with ethidium bromide for sites on the rRNA; under the conditions used in these experiments ethidium bromide is bound to the double-stranded regions of rRNA. 2. When an excess of ethidium bromide is added to ribosomes from Bacillus stearothermophilus approx. 80% of the endogenous spermine is displaced from the ribosomes. 3. [(14)C]Spermine is fixed to ribosomes by either formaldehyde or 1,5-difluoro-2,4-dinitrobenzene. Most of the [(14)C]spermine, fixed to ribosomes by 1,5-difluoro-2,4-dinitrobenzene, attaches to the ribosomal protein. 4. It is concluded that most of the endogenous spermine is bound to the double-stranded RNA in ribosomes, and that some of these double-stranded regions to which spermine is attached also have ribosomal proteins bound to them.
摘要
- 当通过分光光度滴定法、凝胶过滤法或荧光强度变化来测定溴化乙锭在有精胺和无精胺情况下与rRNA的结合时，精胺与溴化乙锭竞争rRNA上的位点；在这些实验所用的条件下，溴化乙锭与rRNA的双链区域结合。2. 当向嗜热脂肪芽孢杆菌的核糖体中加入过量的溴化乙锭时，约80%的内源性精胺会从核糖体上被置换下来。3. [¹⁴C]精胺可通过甲醛或1,5-二氟-2,4-二硝基苯固定到核糖体上。通过1,5-二氟-2,4-二硝基苯固定到核糖体上的大部分[¹⁴C]精胺会附着到核糖体蛋白上。4. 得出的结论是，大部分内源性精胺与核糖体中的双链RNA结合，并且一些与精胺结合的双链区域也结合有核糖体蛋白。
相似文献
1
The location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide.用1,5-二氟-2,4-二硝基苯和溴化乙锭所示精胺在细菌核糖体中的位置。
Biochem J. 1972 Jun;128(2):279-89. doi: 10.1042/bj1280279.
2
The binding of spermine to the ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus.精胺与嗜热脂肪芽孢杆菌核糖体及核糖体核糖核酸的结合
Biochem J. 1969 Jun;113(1):117-21. doi: 10.1042/bj1130117.
3
The binding of polyamines and of ethidium bromide to tRNA.多胺及溴化乙锭与转运核糖核酸（tRNA）的结合
Nucleic Acids Res. 1975 Jul;2(7):1005-22. doi: 10.1093/nar/2.7.1005.
4
Interaction of ethidium bromide with ribosomes. Absorption, fluorescence, circular dichroism and sedimentation studies.溴化乙锭与核糖体的相互作用。吸收、荧光、圆二色性及沉降研究。
Biochim Biophys Acta. 1977 May 3;476(1):65-75. doi: 10.1016/0005-2787(77)90286-6.
5
Binding of magnesium ions and ethidium bromide: comparison of ribosomes and free ribosomal RNA.镁离子与溴化乙锭的结合：核糖体与游离核糖体RNA的比较
Nucleic Acids Res. 1979 Sep 25;7(2):465-80. doi: 10.1093/nar/7.2.465.
6
[Proceedings: Fixation of ethidium bromide to rRNA and to ribosomes of Saccharomyces cerevisiae].[会议论文：溴化乙锭与酿酒酵母rRNA及核糖体的结合]
Arch Int Physiol Biochim. 1974 Oct;82(4):772.
7
Studies on the role of polyamines associated with the ribosomes from Bacillus stearothermophilus.关于与嗜热脂肪芽孢杆菌核糖体相关的多胺作用的研究。
Biochem J. 1968 Jul;108(4):633-40. doi: 10.1042/bj1080633.
8
Specific release of ribosomal proteins by nucleic acid-intercalating agents.核酸嵌入剂对核糖体蛋白的特异性释放
Nucleic Acids Res. 1976 May;3(5):1307-22. doi: 10.1093/nar/3.5.1307.
9
Labilizing action of intercalating drugs and dyes on bacterial ribosomes.
Biochemistry. 1972 Apr 25;11(9):1569-72.
10
Binding of ethidium bromide to 5-S ribosomal RNA.溴化乙锭与5-S核糖体RNA的结合。
Biochim Biophys Acta. 1971 Nov 29;254(1):60-77. doi: 10.1016/0005-2787(71)90114-6.
引用本文的文献
1
Endogenous polyamine function--the RNA perspective.内源性多胺功能——RNA视角
Nucleic Acids Res. 2014 Oct;42(18):11275-90. doi: 10.1093/nar/gku837. Epub 2014 Sep 17.
2
Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions.通过光亲和标记法对23S rRNA中精胺结合位点进行定位：解析精胺对核糖体50S亚基功能的贡献
Nucleic Acids Res. 2005 May 16;33(9):2792-805. doi: 10.1093/nar/gki557. Print 2005.
3
The identification of spermine binding sites in 16S rRNA allows interpretation of the spermine effect on ribosomal 30S subunit functions.在16S核糖体RNA中精胺结合位点的鉴定有助于解释精胺对核糖体30S亚基功能的影响。
Nucleic Acids Res. 2002 Jul 1;30(13):2832-43. doi: 10.1093/nar/gkf404.
4
Multiple transport components for putrescine in Escherichia coli.大肠杆菌中腐胺的多种转运成分。
J Bacteriol. 1974 Jun;118(3):952-63. doi: 10.1128/jb.118.3.952-963.1974.
5
Specific release of ribosomal proteins by nucleic acid-intercalating agents.核酸嵌入剂对核糖体蛋白的特异性释放
Nucleic Acids Res. 1976 May;3(5):1307-22. doi: 10.1093/nar/3.5.1307.
6
Polyamines, ribonucleases, and the stability of RNA.多胺、核糖核酸酶与RNA的稳定性
Mol Cell Biochem. 1977 Sep 9;17(2):89-99. doi: 10.1007/BF01743432.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Measurement of protein-binding phenomena by gel filtration.通过凝胶过滤法测定蛋白质结合现象。
Biochim Biophys Acta. 1962 Oct 8;63:530-2. doi: 10.1016/0006-3002(62)90124-5.
3
Studies on the composition of the protein from Escherichia coli ribosomes.大肠杆菌核糖体蛋白质组成的研究。
Proc Natl Acad Sci U S A. 1961 Jan 15;47(1):18-23. doi: 10.1073/pnas.47.1.18.
4
Fluorescence studies on the 30 S ribosome assembly process.关于30 S核糖体组装过程的荧光研究。
FEBS Lett. 1970 Nov 9;11(1):49-54. doi: 10.1016/0014-5793(70)80489-6.
5
Association of polyamines and RNA in isolated subcellular particles from rat liver.大鼠肝脏分离的亚细胞颗粒中多胺与RNA的关联
Biochim Biophys Acta. 1967 Mar 29;138(1):200-3. doi: 10.1016/0005-2787(67)90604-1.
6
Interaction of spermine and DNA.精胺与DNA的相互作用。
Biopolymers. 1967 Feb;5(2):227-33. doi: 10.1002/bip.1967.360050209.
7
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性
J Mol Biol. 1967 Jul 14;27(1):87-106. doi: 10.1016/0022-2836(67)90353-1.
8
Properties of formaldehyde-treated nucleohistone.甲醛处理的核组蛋白的性质
Biochemistry. 1969 Aug;8(8):3214-8. doi: 10.1021/bi00836a013.
9
The binding of spermine to the ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus.精胺与嗜热脂肪芽孢杆菌核糖体及核糖体核糖核酸的结合
Biochem J. 1969 Jun;113(1):117-21. doi: 10.1042/bj1130117.
10
An X-ray study of the interaction of DNA with spermine.关于DNA与精胺相互作用的X射线研究。
J Mol Biol. 1969 Jun 14;42(2):363-73. doi: 10.1016/0022-2836(69)90049-7.
Zotero 插件