培养的人肿瘤细胞（海拉细胞）中脱氧核糖核酸的生物甲基化。未检测到除5-甲基胞嘧啶以外的甲基化碱基。
Biomethylation of deoxyribonucleic acid in cultured human tumour cells (HeLa). Methylated bases other than 5-methylcytosine not detected.
作者信息
Lawley P D, Crathorn A R, Shah S A, Smith B A
出版信息
Biochem J. 1972 Jun;128(1):133-8. doi: 10.1042/bj1280133.
Abstract
- Incorporation of methyl groups from [methyl-(14)C]methionine into DNA of dividing HeLa cells was investigated, essentially by the procedures of Culp, et al. (1970). 2. Contrary to the report of the latter, but in agreement with other work on biomethylation of mammalian DNA, 5-methylcytosine was the sole methylated base detected. 3. Chromatographic separations of 3-methylcytosine from 5-methylcytosine and purines are discussed.
摘要
- 主要采用卡尔普等人（1970年）的方法，研究了[甲基-（14）C]甲硫氨酸中的甲基基团掺入正在分裂的海拉细胞DNA的情况。2. 与后者的报告相反，但与关于哺乳动物DNA生物甲基化的其他研究一致，5-甲基胞嘧啶是检测到的唯一甲基化碱基。3. 讨论了从5-甲基胞嘧啶和嘌呤中色谱分离3-甲基胞嘧啶的方法。
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