布氏锥虫动质体DNA的大环。

The maxi-circle of Trypanosoma brucei kinetoplast DNA.

作者信息

Borst P, Fase-Fowler F

出版信息

Biochim Biophys Acta. 1979 Nov 22;565(1):1-12. doi: 10.1016/0005-2787(79)90078-9.

Abstract

We have constructed a fragment map of the maxi-circle component from kinetoplast DNA networks of Trypanosoma brucei brucei (East African Trypanosomiasis Research Organization strain No. 427), using restriction endonucleases PstI, HapII, EcoRI, BglI, HindIII, HhaI, SstI, XbaI, HaeIII and Sau-96I. Although the 20 kilo-base pair map contains 30 fragments, there is a 6.5 kilo-base pair 'silent' segment which lacks recognition sites for any of these enzymes. In CsCl intact kinetoplast DNA has a buoyant density of 1.690 g/cm3, whereas a kinetoplast DNA fraction enriched in maxi-circle sequences contains an additional component at 1.682 g/cm3. The possibility that the 'silent' segment is very rich in A + T is discussed.

摘要

我们利用限制性内切酶PstI、HapII、EcoRI、BglI、HindIII、HhaI、SstI、XbaI、HaeIII和Sau-96I构建了布氏布氏锥虫（东非锥虫病研究组织427号菌株）动基体DNA网络中大环成分的片段图谱。尽管20千碱基对的图谱包含30个片段，但有一个6.5千碱基对的“沉默”区段，这些酶中没有一种能识别该区段的位点。在氯化铯中，完整的动基体DNA浮力密度为1.690 g/cm3，而富含大环序列的动基体DNA组分在1.682 g/cm3处还有一个额外的成分。文中讨论了“沉默”区段富含A+T的可能性。