Benne R, De Vries B F, Van den Burg J, Klaver B
Nucleic Acids Res. 1983 Oct 25;11(20):6925-41. doi: 10.1093/nar/11.20.6925.
The nucleotide sequence of a 2.5-kb segment of the maxi-circle of Trypanosoma brucei mtDNA has been determined. The segment contains the gene for apocytochrome b, which displays about 25% homology at the amino acid level to the apocytochrome b gene from fungal and mammalian mtDNAs. Northern blot and S1 nuclease analyses have yielded accurate map positions of an RNA species in an area that coincides with the reading frame. The segment also contains two pairs of overlapping unassigned reading frames, which lack homology with any known mitochondrial gene or URF. The DNA sequence in these areas is AG-rich (70%), resulting in URFs with an unusually high level of glycine and charged amino acids (60%). They may not encode proteins, in spite of their size and the fact that abundant transcripts are mapped in these areas.
已确定布氏锥虫线粒体DNA（mtDNA）大环状分子中一段2.5kb片段的核苷酸序列。该片段包含脱辅基细胞色素b基因，其在氨基酸水平上与真菌和哺乳动物mtDNA中的脱辅基细胞色素b基因约有25%的同源性。Northern印迹和S1核酸酶分析已得出一个RNA种类在与阅读框相符区域的精确图谱位置。该片段还包含两对重叠的未确定阅读框，它们与任何已知的线粒体基因或URF均无同源性。这些区域的DNA序列富含AG（70%），导致URF中甘氨酸和带电荷氨基酸的水平异常高（60%）。尽管它们的大小以及在这些区域有大量转录本定位这一事实，但它们可能不编码蛋白质。
