Popovtzer M M, Massry S G, Villamil M, Kleeman C R
J Clin Invest. 1971 Nov;50(11):2347-54. doi: 10.1172/JCI106733.
The renal handling of phosphorus was evaluated in rats with acute renal failure (ARF) induced by injection of mercuric chloride (HgCl(2)). Clearances of endogenous creatinine (Ccr) and of phosphorus (Cp) were measured in the following groups: 1. Intact animals (control); 2. Parathyroidectomized rats (PTX) with normal kidney function (PTX control); 3. Animals with mercury-induced acute renal failure (Hg-ARF); 4. PTX rats with Hg-ARF; 5. Rats with Hg-ARF maintained normophosphatemic with dietary phosphate restriction; 6. Animals with oliguric ARF following renal artery constriction; 7. Rats with unilateral Hg-ARF. In addition, radioinulin clearances were measured in 6 normal and in 14 azotemic animals and correlated with simultaneously recorded endogenous Ccr. Radioinulin clearance was also used as an estimate of GFR (glomerular filtration rate) in the animals of group 7. The Cp/GFR in the intact animals (group 1) was 0.25 +/-0.06 (mean +/-SD). PTX (group 2) caused a subsequent decrease in Cp/GFR to 0.11 +/-0.04 P < 0.0005. The ARF animals in group 3 were classified either as oliguric (U(vol) [urine volume] <2 ml/24 hr, Ccr 0.008 +/-0.005 ml/min) or nonoliguric (V(vol) >2 ml/24 hr, Ccr 0.136 +/-0.12). The Cp/GFR in the oliguric animals (0.16 +/-0.09) was lower than that in group 1, P < 0.0005, and failed to increase following administration of exogenous parathyroid hormone. The Cp/GFR in the oliguric animals in groups 5 and 7 was also lower than the clearance ratio in group 1, 0.030 +/-0.08 and 0.077 +/-0.006, respectively. In the nonoliguric ARF animals of group 3 the Cp/GFR (0.94 +/-0.29) was higher than that in group 1, P < 0.0005. In the nonoliguric ARF animals of group 4 the Cp/GFR 0.27 +/-0.08 did not differ from the clearance ratio in group 1, however it was higher than that in the PTX animals (group 2) P < 0.0005. Cp/GFR in the nonoliguric animals of group 5 was not different from that in the nonoliguric rats of group 3. In the animals with nonoliguric unilateral Hg-ARF Cp/GFR on the affected side 0.51 +/-0.16 was higher than that on the control (contralateral) side, 0.23 +/-0.07, P < 0.0005. These results indicate that the low Cp/GFR observed in the oliguric ARF animals was not related to the level of circulating parathyroid hormone nor to the presence or absence of azotemia but probably was due to a reduced renal cortical perfusion. The high Cp/GFR in the nonoliguric ARF animals could be explained by secondary hyperparathyroidism and impaired phosphorus reabsorption due to tubular injury.
通过注射氯化汞（HgCl₂）诱导大鼠急性肾衰竭（ARF），评估其肾脏对磷的处理情况。在以下几组中测量内源性肌酐清除率（Ccr）和磷清除率（Cp）：1. 完整动物（对照组）；2. 肾功能正常的甲状旁腺切除大鼠（PTX）（PTX对照组）；3. 汞诱导的急性肾衰竭动物（Hg - ARF）；4. 患有Hg - ARF的PTX大鼠；5. 通过饮食限制磷维持血磷正常的Hg - ARF大鼠；6. 肾动脉狭窄后出现少尿型ARF的动物；7. 单侧Hg - ARF大鼠。此外，在6只正常动物和14只氮质血症动物中测量放射性菊粉清除率，并与同时记录的内源性Ccr相关联。放射性菊粉清除率也被用作第7组动物肾小球滤过率（GFR）的估计值。完整动物（第1组）的Cp/GFR为0.25±0.06（平均值±标准差）。PTX（第2组）导致Cp/GFR随后降至0.11±0.04，P<0.0005。第3组的ARF动物分为少尿型（尿体积[U(vol)]<2 ml/24小时，Ccr 0.008±0.005 ml/分钟）或非少尿型（尿体积[V(vol)]>2 ml/24小时，Ccr 0.136±0.12）。少尿型动物的Cp/GFR（0.16±0.09）低于第1组，P<0.0005，并且在给予外源性甲状旁腺激素后未增加。第5组和第7组少尿型动物的Cp/GFR也低于第1组的清除率比值，分别为0.030±0.08和0.077±0.006。在第3组非少尿型ARF动物中，Cp/GFR（0.94±0.29）高于第1组，P<0.0005。在第4组非少尿型ARF动物中，Cp/GFR为0.27±0.08，与第1组的清除率比值无差异，但高于PTX动物（第2组），P<0.0005。第5组非少尿型动物的Cp/GFR与第3组非少尿型大鼠无差异。在非少尿型单侧Hg - ARF动物中，患侧的Cp/GFR为0.51±0.16，高于对照（对侧）侧的0.23±0.07，P<0.0005。这些结果表明，在少尿型ARF动物中观察到的低Cp/GFR与循环甲状旁腺激素水平无关，也与氮质血症的存在与否无关，但可能是由于肾皮质灌注减少所致。非少尿型ARF动物中高Cp/GFR可以用继发性甲状旁腺功能亢进和肾小管损伤导致的磷重吸收受损来解释。
