Kaloyanides G J, Azer M
J Clin Invest. 1971 Aug;50(8):1603-12. doi: 10.1172/JCI106648.
The role of a humoral mechanism in the natriuresis induced by volume expansion was evaluated using an isolated dog kidney perfused by a second dog which had been pretreated with desoxycorticosterone acetate (DOCA). Expansion of the perfusion dog with an equilibrated volume of blood from a reservoir, resulted in an increase in UnaV (sodium excretion) from 153.6+/-27.9 (sem) to 345.5+/-57.8 muEq/min, P<0.001. FEna (fractional sodium excretion) increased from 3.4+/-0.6 to 8.1+/-1.2%, P<0.01. The natriuresis occurred in the face of a significant decrease in Cin, RBF, and renal arterial pressure, and in the absence of any change in plasma protein concentration or packed cell volume. In a control group of experiments, sodium excretion did not change when the perfusion dog was not volume expanded, although Cin (inulin clearance) and RBF (renal blood flow) decreased to the same degree as in the expanded group. These data support the conclusion that volume expansion of the perfusion dog either stimulated the release of a natriuretic factor or suppressed the release of an antinatriuretic factor which was manifested by an increase in sodium excretion in the isolated kidney.
利用一只经醋酸脱氧皮质酮（DOCA）预处理的犬对另一只犬的离体肾脏进行灌注，以此评估体液机制在容量扩张诱导的利钠作用中的作用。用储液器中平衡体积的血液使灌注犬容量扩张后，尿钠排泄量（UnaV）从153.6±27.9（标准误）增加至345.5±57.8μEq/分钟，P<0.001。滤过钠排泄分数（FEna）从3.4±0.6增加至8.1±1.2%，P<0.01。利钠作用发生在菊粉清除率（Cin）、肾血流量（RBF）和肾动脉压显著降低的情况下，且血浆蛋白浓度或红细胞压积未发生任何变化。在一组对照实验中，当灌注犬未进行容量扩张时，钠排泄量未发生变化，尽管菊粉清除率（Cin）和肾血流量（RBF）下降程度与容量扩张组相同。这些数据支持以下结论：灌注犬的容量扩张要么刺激了利钠因子的释放，要么抑制了抗利钠因子的释放，这表现为离体肾脏中钠排泄增加。
