Haupert G T, Sancho J M
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4658-60. doi: 10.1073/pnas.76.9.4658.
A low molecular weight, basic, nonpeptidic factor that possesses sodium transport inhibitory properties has been prepared from bovine hypothalamus by acid/acetone extraction and gel filtration. Concentration and partial purification was achieved by ion-exchange chromatography. This substance inhibits active Na+ transport across anuran membranes, inhibits ouabain binding to frog urinary bladder, and directly inhibits renal Na+,K+-ATPase. This substance thus possesses the putative characteristics of a natriuretic factor of hypothalamic origin.
一种具有钠转运抑制特性的低分子量、碱性、非肽类因子已通过酸/丙酮提取和凝胶过滤从牛下丘脑制备出来。通过离子交换色谱法实现了浓缩和部分纯化。该物质抑制无尾两栖类动物膜上的活性Na⁺转运，抑制哇巴因与青蛙膀胱的结合，并直接抑制肾Na⁺,K⁺-ATP酶。因此，该物质具有下丘脑来源的利钠因子的假定特征。
