分离的牛甲状腺细胞核和核仁中的RNA合成。α-鹅膏蕈碱效应，提示存在特定调节系统。

RNA synthesis in isolated bovine thyroid nuclei and nucleoli. alpha-Amanitin effect, a hint to the existence of a specific regulatory system.

作者信息

Voets R, Lagrou A, Hilderson H, Van Dessel G, Dierick W

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Sep;360(9):1271-83. doi: 10.1515/bchm2.1979.360.2.1271.

DOI:10.1515/bchm2.1979.360.2.1271

Abstract

DNA dependent RNA polymerase activities in isolated bovine thyroid nuclei and nucleoli have been studied. They retain their RNA synthetic activity for an extended period of time. This RNA synthetic activity is sensitive to actinomycin D and requires the presence of all four ribonucleoside triphosphates. The optimal conditions have been determined. Polyacrylamide gel electrophoresis reveals that the RNA synthesized has a size distribution ranging from 34S to 4S. The production of 18S-8S RNA is very sensitive to low concentrations of alpha-amanitin. However, in isolated bovine thyroid nuclei (not in nucleoli) this drug displays an effect on all RNA classes produced. The alpha-amanitin induced drastic decrease of [3H]-UMP incorporation in RNA of all sizes synthesized by isolated bovine thyroid nuclei is discussed.

摘要

对分离出的牛甲状腺细胞核和核仁中的DNA依赖性RNA聚合酶活性进行了研究。它们在较长一段时间内保持其RNA合成活性。这种RNA合成活性对放线菌素D敏感，并且需要所有四种核糖核苷三磷酸的存在。已确定了最佳条件。聚丙烯酰胺凝胶电泳显示，合成的RNA的大小分布范围为34S至4S。18S - 8S RNA的产生对低浓度的α-鹅膏蕈碱非常敏感。然而，在分离出的牛甲状腺细胞核中（而非核仁中），这种药物对所产生的所有RNA类别都有影响。讨论了α-鹅膏蕈碱诱导的由分离出的牛甲状腺细胞核合成的各种大小RNA中[3H]-UMP掺入量的急剧下降。