从大鼠睾丸的生精小管中分离生殖细胞高尔基体。
Isolation of germ cell Golgi apparatus from seminiferous tubules of rat testes.
作者信息
Cunningham W P, Mollenhauer H H, Nyquist S E
出版信息
J Cell Biol. 1971 Oct;51(1):273-85. doi: 10.1083/jcb.51.1.273.
Intact Golgi apparatus have been isolated with good purity from rat testis by a simplified sucrose gradient technique. The procedure is inherently selective in that most of the Golgi apparatus in the isolate are from germ cells in late spermatocyte or early spermatid development. No Sertoli cell Golgi apparatus are present in the fraction. Biochemical analyses showed that the enzyme N-acetylglucosamine galactosyltransferase is enhanced in the band containing Golgi apparatus. Because they are easy to isolate, and because they are involved with the formation of a specific internal cellular component (the acrosome), these Golgi apparatus will be useful objects for comparison with other kinds of Golgi apparatus and for other studies leading to a better understanding of the basic functioning of Golgi apparatus.
通过一种简化的蔗糖梯度技术，已从大鼠睾丸中分离出纯度良好的完整高尔基体。该方法具有内在的选择性，即分离物中的大部分高尔基体来自精子细胞发育后期或精子细胞早期的生殖细胞。该组分中不存在支持细胞高尔基体。生化分析表明，含高尔基体的条带中N-乙酰葡糖胺半乳糖基转移酶活性增强。由于它们易于分离，并且由于它们参与特定细胞内成分（顶体）的形成，这些高尔基体将成为与其他种类高尔基体进行比较以及用于其他研究以更好地理解高尔基体基本功能的有用对象。
Zotero 插件