在非洲爪蟾卵母细胞翻译系统中合成的大鼠免疫球蛋白的翻译后修饰。
Post-translational modification of rat immunoglobulins synthesized in the Xenopus oocyte translation system.
作者信息
Deacon N J, Ebringer A
出版信息
Immunology. 1979 Sep;38(1):137-44.
The post-translational modification of rat immunoglobulin synthesised in Xenopus laevis oocytes was studied. The major products of translation of rat spleen poly-(A) containing mRNA were found to be assembled 7S immunoglobulin molecules indicating extensive modification of primary translation products. The possibility that these immunoglobulin molecules might include antibodies of defined specificity was investigated using spleen mRNA from rats hyperimmunized with ferritin and keyhole limpet haemocyanin. The presence of antibodies to immunizing antigen in oocyte translation products was determined by affinity chromatography on Sepharose-antigen columns and the synthesis of Sepharose-antigen binding antibodies was observed, equivalent to 2.5-3% of total immunoglobulins. The oocyte produced antibodies were of the same immunoglobulin class as the circulating antibodies from the immunized rats.
对在非洲爪蟾卵母细胞中合成的大鼠免疫球蛋白的翻译后修饰进行了研究。发现大鼠脾脏含多聚腺苷酸（poly - A）的mRNA翻译的主要产物是组装好的7S免疫球蛋白分子，这表明初级翻译产物发生了广泛修饰。使用经铁蛋白和钥孔戚血蓝蛋白超免疫的大鼠的脾脏mRNA，研究了这些免疫球蛋白分子可能包含具有特定特异性抗体的可能性。通过在琼脂糖 - 抗原柱上进行亲和层析来确定卵母细胞翻译产物中针对免疫抗原的抗体的存在，并且观察到了琼脂糖 - 抗原结合抗体的合成，相当于总免疫球蛋白的2.5 - 3%。卵母细胞产生的抗体与免疫大鼠的循环抗体属于同一免疫球蛋白类别。
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