大鼠体内氧代卟啉及其高铁血红素转化为胆色素的同位素研究。
Isotopic studies of the conversion of oxophlorins and their ferrihaems into bile pigments in the rat.
作者信息
Kondo T, Nicholson D C, Jackson A H, Kenner G W
出版信息
Biochem J. 1971 Feb;121(4):601-7. doi: 10.1042/bj1210601.
Abstract
- Tritiated oxymesoporphyrins and their ferrihaems were tested as possible intermediates in the catabolism of haemoglobin. The tritiated compounds were injected into rats with biliary fistulae and the incorporation of the isotope into bile, bile pigment, urine, faeces, liver, kidney and spleen was measured. 2. alpha-Oxymesoferrihaem was extensively converted into bile pigment and specifically to the expected mesobilirubin. 3. beta-Oxymesoferrihaem was poorly converted into bile pigment and was not converted into mesobiliverdin IXbeta. The latter was independently shown to be excreted rapidly in bile. 4. The free oxyporphyrins were also poor precursors of bile pigment, and alpha-oxymesoporphyrin competed with bilirubin for excretion by the liver. 5. By analogy with the results obtained with alpha-oxymesoferrihaem it is concluded that alpha-oxyprotoferrihaem is an intermediate in the catabolism of haemoglobin, undergoing further oxidation to bile pigment under the catalysis of an enzyme of definite specificity.
摘要
- 用氚标记的氧化中卟啉及其高铁血红素作为血红蛋白分解代谢中可能的中间体进行了测试。将这些氚标记的化合物注射到患有胆瘘的大鼠体内，然后测定同位素在胆汁、胆色素、尿液、粪便、肝脏、肾脏和脾脏中的掺入情况。2. α-氧化中高铁血红素大量转化为胆色素，特别是转化为预期的中胆红素。3. β-氧化中高铁血红素很少转化为胆色素，也不转化为中胆绿素IXβ。后者被独立证明可迅速经胆汁排泄。4. 游离的氧化卟啉也是胆色素的不良前体，α-氧化中卟啉与胆红素竞争经肝脏排泄。5. 根据用α-氧化中高铁血红素获得的结果类推，得出结论：α-氧化原高铁血红素是血红蛋白分解代谢的中间体，在具有特定特异性的酶的催化下进一步氧化为胆色素。
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