极端嗜盐细菌的核酸酶学。深红嗜盐杆菌核糖核酸依赖性核糖核酸聚合酶。
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum ribonucleic acid-dependent ribonucleic acid polymerase.
作者信息
Louis B G, Fitt P S
出版信息
Biochem J. 1971 Feb;121(4):629-33. doi: 10.1042/bj1210629.
Abstract
- Crude extracts of the extreme halophile Halobacterium cutirubrum contain separable DNA-dependent and RNA-dependent RNA polymerases. 2. The RNA-dependent enzyme has been purified about 2800-fold. 3. It requires RNA, preferably of high molecular weight, and all four ribonucleoside triphosphates to incorporate (14)C-labelled nucleoside triphosphate into an acid-insoluble, ribonuclease-sensitive product. 4. Both the stability and activity of the RNA polymerase are relatively insensitive to changes in potassium chloride or sodium chloride concentration, but incorporation is stimulated by both Mg(2+) and Mn(2+). 5. The molecular weight of the enzyme is about 17000-18000.
摘要
- 极端嗜盐菌红皮盐杆菌的粗提物含有可分离的依赖DNA的RNA聚合酶和依赖RNA的RNA聚合酶。2. 依赖RNA的酶已被纯化了约2800倍。3. 它需要RNA，最好是高分子量的RNA，以及所有四种核糖核苷三磷酸，才能将（14）C标记的核苷三磷酸掺入酸不溶性、对核糖核酸酶敏感的产物中。4. RNA聚合酶的稳定性和活性对氯化钾或氯化钠浓度的变化相对不敏感，但镁离子（Mg2+）和锰离子（Mn2+）都能刺激掺入过程。5. 该酶的分子量约为17000 - 18000。
相似文献
1
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum ribonucleic acid-dependent ribonucleic acid polymerase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌核糖核酸依赖性核糖核酸聚合酶。
Biochem J. 1971 Feb;121(4):629-33. doi: 10.1042/bj1210629.
2
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌依赖脱氧核糖核酸的核糖核酸聚合酶。
Biochem J. 1971 Feb;121(4):621-7. doi: 10.1042/bj1210621.
3
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum polynucleotide phosphorylase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌多核苷酸磷酸化酶。
Biochem J. 1971 Feb;121(4):613-20. doi: 10.1042/bj1210613.
4
Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases.极端嗜盐菌的核酸酶学。嗜盐栖热放线菌多核苷酸磷酸化酶以及依赖脱氧核糖核酸和核糖核酸的核糖核酸聚合酶分子量的凝胶过滤和密度梯度离心研究。
Biochem J. 1971 Feb;121(4):635-41. doi: 10.1042/bj1210635.
5
Isolation and properties of highly purified Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.高度纯化的深红嗜盐菌脱氧核糖核酸依赖性核糖核酸聚合酶的分离与特性
Biochem J. 1972 Mar;127(1):69-80. doi: 10.1042/bj1270069.
6
Purification and properties of the ribonucleic acid-dependent ribonucleic acid polymerase from Halobacterium cutirubrum.来自红皮盐杆菌的核糖核酸依赖性核糖核酸聚合酶的纯化及性质
Biochem J. 1972 Jul;128(4):755-62. doi: 10.1042/bj1280755.
7
Some properties of a DNA-dependent RNA polymerase from Halobacterium cutirubrum.来自红皮盐杆菌的一种依赖DNA的RNA聚合酶的某些特性。
Can J Biochem. 1973 Sep;51(9):1297-304. doi: 10.1139/o73-170.
8
The role of Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase subunits in initiation and polymerization.深红嗜盐菌脱氧核糖核酸依赖性核糖核酸聚合酶亚基在起始和聚合过程中的作用。
Biochem J. 1972 Mar;127(1):81-6. doi: 10.1042/bj1270081.
9
Superoxide dismutase from the extremely halophilic archaebacterium Halobacterium cutirubrum.来自极端嗜盐古细菌红皮盐杆菌的超氧化物歧化酶。
J Bacteriol. 1987 Apr;169(4):1417-22. doi: 10.1128/jb.169.4.1417-1422.1987.
10
Effect of monovalent cations on the malic enzyme from the extreme halophile, Halobacterium cutirubrum.单价阳离子对极端嗜盐菌——红皮盐杆菌苹果酸酶的影响。
J Bacteriol. 1972 Jan;109(1):437-9. doi: 10.1128/jb.109.1.437-439.1972.
引用本文的文献
1
Evaluation of Strain R4 Capabilities for Cadmium Removal from Brines.评估 R4 菌株从卤水中去除镉的能力。
Mar Drugs. 2023 Jan 21;21(2):72. doi: 10.3390/md21020072.
2
Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases.极端嗜盐菌的核酸酶学。嗜盐栖热放线菌多核苷酸磷酸化酶以及依赖脱氧核糖核酸和核糖核酸的核糖核酸聚合酶分子量的凝胶过滤和密度梯度离心研究。
Biochem J. 1971 Feb;121(4):635-41. doi: 10.1042/bj1210635.
3
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum polynucleotide phosphorylase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌多核苷酸磷酸化酶。
Biochem J. 1971 Feb;121(4):613-20. doi: 10.1042/bj1210613.
4
Isolation and properties of highly purified Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.高度纯化的深红嗜盐菌脱氧核糖核酸依赖性核糖核酸聚合酶的分离与特性
Biochem J. 1972 Mar;127(1):69-80. doi: 10.1042/bj1270069.
5
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌依赖脱氧核糖核酸的核糖核酸聚合酶。
Biochem J. 1971 Feb;121(4):621-7. doi: 10.1042/bj1210621.
6
Purification and properties of the ribonucleic acid-dependent ribonucleic acid polymerase from Halobacterium cutirubrum.来自红皮盐杆菌的核糖核酸依赖性核糖核酸聚合酶的纯化及性质
Biochem J. 1972 Jul;128(4):755-62. doi: 10.1042/bj1280755.
7
Salt-dependent properties of proteins from extremely halophilic bacteria.极端嗜盐细菌蛋白质的盐依赖性特性
Bacteriol Rev. 1974 Sep;38(3):272-90. doi: 10.1128/br.38.3.272-290.1974.
8
The relationship between the deoxyribonucleic acid-bound and low-molecular-weight soluble forms of Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.深红嗜盐菌脱氧核糖核酸依赖性核糖核酸聚合酶的脱氧核糖核酸结合形式与低分子量可溶形式之间的关系。
Biochem J. 1975 Sep;149(3):719-24. doi: 10.1042/bj1490719.
9
Microbial water stress.微生物水胁迫
Bacteriol Rev. 1976 Dec;40(4):803-46. doi: 10.1128/br.40.4.803-846.1976.
10
Isolation and properties of a small manganese-ion-stimulated bacterial alkaline phosphatase.一种受锰离子刺激的小型细菌碱性磷酸酶的分离与特性
Biochem J. 1976 Jul 1;157(1):161-7. doi: 10.1042/bj1570161.
本文引用的文献
1
ENZYMIC SYNTHESIS OF RNA WITH REOVIRUS RNA AS TEMPLATE. I. CHARACTERISTICS OF THE REACTION CATALYZED BY THE RNA POLYMERASE FROM ESCHERICHIA COLI.以呼肠孤病毒RNA为模板的RNA酶促合成。I. 大肠杆菌RNA聚合酶催化反应的特性
J Mol Biol. 1964 Jul;9:193-207. doi: 10.1016/s0022-2836(64)80100-5.
2
3
4
Mechanism of synthesis of bacteriophage RNA.噬菌体RNA的合成机制。
Nature. 1969 Apr 12;222(5189):121-3. doi: 10.1038/222121a0.
5
[Demonstration of an RNA-dependent RNA replicase from immunologically competent cells by micro disk electrophoresis].[通过微量圆盘电泳从免疫活性细胞中证明一种依赖RNA的RNA复制酶]
Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):157-62.
6
The mechanism of RNA replication.RNA复制的机制。
Cold Spring Harb Symp Quant Biol. 1968;33:101-24. doi: 10.1101/sqb.1968.033.01.015.
7
Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases.极端嗜盐菌的核酸酶学。嗜盐栖热放线菌多核苷酸磷酸化酶以及依赖脱氧核糖核酸和核糖核酸的核糖核酸聚合酶分子量的凝胶过滤和密度梯度离心研究。
Biochem J. 1971 Feb;121(4):635-41. doi: 10.1042/bj1210635.
8
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum polynucleotide phosphorylase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌多核苷酸磷酸化酶。
Biochem J. 1971 Feb;121(4):613-20. doi: 10.1042/bj1210613.
9
Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum deoxyribonucleic acid-dependent ribonucleic acid polymerase.极端嗜盐细菌的核酸酶学。深红嗜盐杆菌依赖脱氧核糖核酸的核糖核酸聚合酶。
Biochem J. 1971 Feb;121(4):621-7. doi: 10.1042/bj1210621.
Zotero 插件