极端嗜盐细菌的核酸酶学。深红嗜盐杆菌核糖核酸依赖性核糖核酸聚合酶。

Biochem J. 1971 Feb;121(4):629-33. doi: 10.1042/bj1210629.

Crude extracts of the extreme halophile Halobacterium cutirubrum contain separable DNA-dependent and RNA-dependent RNA polymerases. 2. The RNA-dependent enzyme has been purified about 2800-fold. 3. It requires RNA, preferably of high molecular weight, and all four ribonucleoside triphosphates to incorporate (14)C-labelled nucleoside triphosphate into an acid-insoluble, ribonuclease-sensitive product. 4. Both the stability and activity of the RNA polymerase are relatively insensitive to changes in potassium chloride or sodium chloride concentration, but incorporation is stimulated by both Mg(2+) and Mn(2+). 5. The molecular weight of the enzyme is about 17000-18000.

极端嗜盐菌红皮盐杆菌的粗提物含有可分离的依赖DNA的RNA聚合酶和依赖RNA的RNA聚合酶。2. 依赖RNA的酶已被纯化了约2800倍。3. 它需要RNA，最好是高分子量的RNA，以及所有四种核糖核苷三磷酸，才能将（14）C标记的核苷三磷酸掺入酸不溶性、对核糖核酸酶敏感的产物中。4. RNA聚合酶的稳定性和活性对氯化钾或氯化钠浓度的变化相对不敏感，但镁离子（Mg2+）和锰离子（Mn2+）都能刺激掺入过程。5. 该酶的分子量约为17000 - 18000。

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核心技术专利：CN118964589B侵权必究

Biochem J. 1971 Feb;121(4):629-33. doi: 10.1042/bj1210629.

Crude extracts of the extreme halophile Halobacterium cutirubrum contain separable DNA-dependent and RNA-dependent RNA polymerases. 2. The RNA-dependent enzyme has been purified about 2800-fold. 3. It requires RNA, preferably of high molecular weight, and all four ribonucleoside triphosphates to incorporate (14)C-labelled nucleoside triphosphate into an acid-insoluble, ribonuclease-sensitive product. 4. Both the stability and activity of the RNA polymerase are relatively insensitive to changes in potassium chloride or sodium chloride concentration, but incorporation is stimulated by both Mg(2+) and Mn(2+). 5. The molecular weight of the enzyme is about 17000-18000.

极端嗜盐菌红皮盐杆菌的粗提物含有可分离的依赖DNA的RNA聚合酶和依赖RNA的RNA聚合酶。2. 依赖RNA的酶已被纯化了约2800倍。3. 它需要RNA，最好是高分子量的RNA，以及所有四种核糖核苷三磷酸，才能将（14）C标记的核苷三磷酸掺入酸不溶性、对核糖核酸酶敏感的产物中。4. RNA聚合酶的稳定性和活性对氯化钾或氯化钠浓度的变化相对不敏感，但镁离子（Mg2+）和锰离子（Mn2+）都能刺激掺入过程。5. 该酶的分子量约为17000 - 18000。