Gotoh H, Murota M, Kamiyama A
Histochemistry. 1979 Oct;63(3):323-8. doi: 10.1007/BF00490060.
An improved laser microprobe procedure is developed and applied to the measurement of calcium content in microareas of right and left subepicardial muscles. The staining of canine cardiac muscles by Methylene Blue solution (1% w/v) was found to improve sampling efficiency. Elemental content is proportional to T-1/gamma, where T is the transmittance of the characteristic emission line of the element of the photographic plate and gamma is its contrast. In the present system, the calcium content is analyzed using T-3.2. We find that the staining of samples and the determination of T-1/gamma are useful procedures in the application of laser microprobe to the study of elemental content in biologic microareas.
开发了一种改进的激光微探针程序，并将其应用于测量左右心外膜下肌肉微区的钙含量。发现用亚甲蓝溶液（1% w/v）对犬心肌进行染色可提高采样效率。元素含量与T-1/γ成正比，其中T是照相板上元素特征发射线的透光率，γ是其对比度。在本系统中，使用T-3.2分析钙含量。我们发现，样品染色和T-1/γ的测定是激光微探针应用于生物微区元素含量研究的有用程序。
