通过用四硝基甲烷处理使酪氨酸交联。
The cross-linking of tyrosine by treatment with tetranitromethane.
作者信息
Williams J, Lowe J M
出版信息
Biochem J. 1971 Jan;121(2):203-9. doi: 10.1042/bj1210203.
Abstract
- Tyrosine was treated with tetranitromethane. 2. Approx. 10% of the tyrosine was converted into 3-nitrotyrosine. 3. Three fluorescent compounds were also formed. They appear to be a dimer, trimer and tetramer in which tyrosine units are linked by biphenyl bonds. 4. The dimer and trimer have also been isolated from some proteins after treatment with tetranitromethane. 5. The yield of 3-nitrotyrosine from ovotransferrin after treatment with tetranitromethane was much smaller than the loss of tyrosine. 6. Several unidentified compounds were also formed by the reaction between tyrosine and tetranitromethane.
摘要
- 酪氨酸用四硝基甲烷处理。2. 约10%的酪氨酸转化为3-硝基酪氨酸。3. 还形成了三种荧光化合物。它们似乎是二聚体、三聚体和四聚体，其中酪氨酸单元通过联苯键相连。4. 用四硝基甲烷处理后，二聚体和三聚体也已从一些蛋白质中分离出来。5. 用四硝基甲烷处理后，卵转铁蛋白中3-硝基酪氨酸的产率远低于酪氨酸的损失量。6. 酪氨酸与四硝基甲烷反应还形成了几种未鉴定的化合物。
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