Podoler H, Applebaum S W
Biochem J. 1971 Jan;121(2):317-20. doi: 10.1042/bj1210317.
Callosobruchus chinensis larval amylase was isolated and purified in five steps, which included co-precipitation with glycogen and column chromatography on ECTEOLA-cellulose. The enzyme was homogeneous by disc gel electrophoresis on polyacrylamide. The alpha-amylase nature was evidenced by the action on amylopectin beta-amylase limit-dextrin, by the effect on the substrate-iodine complex and by the action pattern on several polysaccharide substrates. These action patterns are compared with those of other alpha-amylases.
绿豆象幼虫淀粉酶经过五个步骤进行分离和纯化，包括与糖原共沉淀以及在ECTEOLA-纤维素上进行柱色谱分离。通过聚丙烯酰胺圆盘凝胶电泳证明该酶是均一的。通过对支链淀粉β-淀粉酶极限糊精的作用、对底物-碘复合物的影响以及对几种多糖底物的作用模式，证明了该酶具有α-淀粉酶的性质。将这些作用模式与其他α-淀粉酶的作用模式进行了比较。
