• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

A study of heat of formation of acetyl-alpha-chymotrypsin intermediate by stopped flow calorimetry.

作者信息

Nakamura T

出版信息

J Biochem. 1979 Sep;86(3):751-5. doi: 10.1093/oxfordjournals.jbchem.a132580.

DOI:10.1093/oxfordjournals.jbchem.a132580
PMID:511846
Abstract

The catalytic hydrolysis of p-nitrophenyl acetate by alpha-chymotrypsin was studied by stopped-flow calorimetry and spectrophotometry at pH 8.0 and 25 degrees C. The initial burst and subsequent steady state of the reaction were observed by rapid calorimetry and spectrophotometry. Based on the three-step mechanism established for the enzymatic reaction, E + S in equilibrium ES leads to acetyl-E + P1 (p-nitrophenol) leads to E + P1 + acetic acid, the enthalpy change of formation of the acetyl enzyme from ES complex was estimated to be -29 kJ . mol-1.

摘要

相似文献

1
A study of heat of formation of acetyl-alpha-chymotrypsin intermediate by stopped flow calorimetry.
J Biochem. 1979 Sep;86(3):751-5. doi: 10.1093/oxfordjournals.jbchem.a132580.
2
[Study of the enzymatic hydrolysis of a phosphonic ester using microcalorimetry].[使用微量量热法对膦酸酯的酶促水解进行的研究]
Biochimie. 1979;61(9):1091-4.
3
Modification of isoleucine-16 acetylated delta-chymotrypsin.异亮氨酸-16乙酰化δ-胰凝乳蛋白酶的修饰
J Biol Chem. 1975 Jul 25;250(14):5393-9.
4
Rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin G acylase from Kluyvera citrophila. Effects of mutation F360V on rate constants for acylation and de-acylation.嗜柠檬酸克鲁维酵母青霉素G酰化酶催化乙酸对硝基苯酯水解的快速爆发动力学。F360V突变对酰化和去酰化速率常数的影响。
Biochem J. 1996 Jun 1;316 ( Pt 2)(Pt 2):409-12. doi: 10.1042/bj3160409.
5
Pressure effects on substrate activation phenomena in the alpha-chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate.压力对α-胰凝乳蛋白酶催化对硝基苯乙酸酯水解中底物活化现象的影响。
Biochim Biophys Acta. 1988 Jun 13;954(3):343-6. doi: 10.1016/0167-4838(88)90089-1.
6
Thermodynamics of the binding of Streptomyces subtilisin inhibitor to alpha-chymotrypsin.链霉菌枯草杆菌蛋白酶抑制剂与α-糜蛋白酶结合的热力学
Biochemistry. 1985 Sep 10;24(19):5109-15. doi: 10.1021/bi00340a023.
7
Probing the role of tightly bound phosphoenolpyruvate in Escherichia coli 3-deoxy-d-manno-octulosonate 8-phosphate synthase catalysis using quantitative time-resolved electrospray ionization mass spectrometry in the millisecond time range.利用毫秒时间范围内的定量时间分辨电喷雾电离质谱法探究紧密结合的磷酸烯醇丙酮酸在大肠杆菌3-脱氧-D-甘露糖辛酮酸酯8-磷酸合酶催化中的作用。
Anal Biochem. 2005 Aug 1;343(1):35-47. doi: 10.1016/j.ab.2005.04.021.
8
Kinetics of alpha-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants.α-胰凝乳蛋白酶在乙醇胺表面活性剂中催化乙酸对硝基苯酯水解的动力学
Indian J Biochem Biophys. 2008 Oct;45(5):350-3.
9
Synthesis and characterization of a selenium-containing substrate of alpha-chymotrypsin. Selenium-77 nuclear magnetic resonance observation of an acyl-alpha-chymotrypsin intermediate.α-胰凝乳蛋白酶含硒底物的合成与表征。酰基-α-胰凝乳蛋白酶中间体的硒-77核磁共振观察。
Biochemistry. 1986 Sep 23;25(19):5625-32. doi: 10.1021/bi00367a042.
10
Specificity of -chymotrypsin. Separation of polar, steric, and specific effects in the -chymotrypsin-catalyzed hydrolysis of acyl-substituted p-nitrophenyl esters.胰凝乳蛋白酶的特异性。在胰凝乳蛋白酶催化的酰基取代对硝基苯酯水解反应中极性、空间和特异性效应的分离。
Biochemistry. 1973 Jul 3;12(14):2559-66. doi: 10.1021/bi00738a002.