Wong R S, Scarborough G A, Borek E
J Bacteriol. 1971 Oct;108(1):446-50. doi: 10.1128/jb.108.1.446-450.1971.
Transfer ribonucleic acid (tRNA) methylases were studied during the germination of spores in Neurospora crassa. The total methylase capacity and base specific tRNA methylase activities were determined in extracts from cells harvested at various stages of germination. Germinated conidia have a 65% higher methylase capacity than ungerminated conidia. Three predominant methylase activities were found in the extracts, and the relative amount of each activity was different at the various stages. Enzymes from vegetative cells catalyzed significant hypermethylation of tRNA from conidia, whereas conidial enzymes were much less active on tRNA from vegetative cells. The results indicate differences in the tRNA methylase content and tRNA species of conidia and vegetative cells.
在粗糙脉孢菌孢子萌发过程中对转移核糖核酸（tRNA）甲基化酶进行了研究。在孢子萌发不同阶段收获的细胞提取物中测定了总甲基化酶活性及碱基特异性tRNA甲基化酶活性。已萌发的分生孢子的甲基化酶活性比未萌发的分生孢子高65%。提取物中发现了三种主要的甲基化酶活性，且每种活性的相对含量在不同阶段有所不同。来自营养细胞的酶催化分生孢子tRNA发生显著的超甲基化，而分生孢子的酶对营养细胞的tRNA活性则低得多。结果表明分生孢子和营养细胞的tRNA甲基化酶含量及tRNA种类存在差异。
