Smith R E, Dean P N
J Histochem Cytochem. 1979 Nov;27(11):1499-504. doi: 10.1177/27.11.512332.
A brief historical review of cytoenzymology is presented from the time of introduction into electron microscopy to the present, where the direction for quantification of an enzyme in single cells appears most promising by fluorescent staining. First attempts are reported to quantitate acid phosphatase (AcPase) and dipeptidyl aminopeptidase II (DAP-II) in monodispersed anterior pituitary cells from lactating and postlactating rats by flow cytometry, fluorescent, and electron microscopy. 3-Hydroxy-flavone is introduced as a new fluorescent cytochemical stain for AcPase, useful in flow cytometry but of only limited use in fluorescent microscopy. Histograms for AcPase indicate a single peak of cells staining more intensely in cell preparations from postlactating over lactating animals. Histograms for DAP-II staining indicate two distinct populations of cells present in the lactating and only one in the postlactating rat anterior pituitary gland. The application of dual laser staining indicates that not all cells stain for both enzymes. Electron microscopy shows the subcellular localization of DAP-II to be limited to lytic bodies and in mammotrophic cells to some secretion granules.
本文简要回顾了细胞酶学从引入电子显微镜至今的历史，目前通过荧光染色对单细胞中的酶进行定量分析的方向看起来最有前景。报告了首次尝试通过流式细胞术、荧光显微镜和电子显微镜对来自泌乳和泌乳后大鼠的单分散垂体前叶细胞中的酸性磷酸酶（AcPase）和二肽基氨基肽酶II（DAP-II）进行定量。引入3-羟基黄酮作为AcPase的一种新的荧光细胞化学染色剂，它在流式细胞术中有用，但在荧光显微镜中用途有限。AcPase的直方图表明，与泌乳动物相比，泌乳后动物细胞制剂中染色更强的细胞有一个单峰。DAP-II染色的直方图表明，泌乳大鼠垂体前叶存在两个不同的细胞群体，而泌乳后大鼠垂体前叶只有一个细胞群体。双激光染色的应用表明，并非所有细胞都对这两种酶染色。电子显微镜显示，DAP-II的亚细胞定位仅限于溶酶体，在促乳腺细胞中则定位于一些分泌颗粒。
