Müller E, Pearse A G, Moss D W
Biochem J. 1971 Aug;123(5):895-900. doi: 10.1042/bj1230895.
Subcutaneous injection of isoprenaline into rats produces a rapid rise in alkaline phosphatase activity which is largely confined to the right atrium of the heart. The phosphatase activity of extracts of atrial tissue from treated animals is on average four times that of controls. The rise in activity is largely suppressed by previously treating the animals with cycloheximide or actinomycin D. Comparison of the properties of the alkaline phosphatase in extracts of atria from isoprenaline-treated rats with control preparations shows the stimulated enzyme activity to be similar to that present in the resting state. In a number of respects, including substrate specificity, response to activators and inhibitors, rate of loss of activity on heating and electrophoretic mobility of the native and modified enzyme, the atrial phosphatase resembles alkaline phosphatases from other mammalian non-intestinal and non-placental tissues. These results suggest that the effect of isoprenaline is to increase the rate of synthesis of atrial alkaline phosphatase, and the ability of the tissues of the right atrium to respond in this way may be related to the rich sympathetic innervation of this part of the heart.
给大鼠皮下注射异丙肾上腺素会使碱性磷酸酶活性迅速升高，这种升高主要局限于心脏的右心房。经处理动物的心房组织提取物的磷酸酶活性平均是对照动物的四倍。预先用环己酰亚胺或放线菌素D处理动物，可在很大程度上抑制活性的升高。将异丙肾上腺素处理的大鼠心房提取物中的碱性磷酸酶特性与对照制剂进行比较，结果显示受刺激的酶活性与静息状态下的酶活性相似。在许多方面，包括底物特异性、对激活剂和抑制剂的反应、加热时活性丧失的速率以及天然和修饰酶的电泳迁移率，心房磷酸酶类似于其他哺乳动物非肠道和非胎盘组织中的碱性磷酸酶。这些结果表明，异丙肾上腺素的作用是提高心房碱性磷酸酶的合成速率，右心房组织以这种方式做出反应的能力可能与该部分心脏丰富的交感神经支配有关。
