Chowdhury A K
J Endocrinol. 1979 Sep;82(3):331-40. doi: 10.1677/joe.0.0820331.
Quantitative analysis of spermatogenesis was used to investigate the dependence of testicular germ cells on hormones in Sprague-Dawley rats. Adult hypophysectomized rats were given daily injections of testosterone propionate (TP) for 30 days. Intact and hypophysectomized control rats received vehicle only. Spermatogonia were classified as undifferentiated or differentiated, using established criteria suitable for morphological identification on periodic acid-Schiff's-haematoxylin stained sections of testis. Data on cell counts showed that the undifferentiated spermatogonia may be partially dependent on TP and/or pituitary hormones. The group of differentiated spermatogonia were dependent on pituitary hormones, and TP only partially restored their number by partially protecting them from spontaneous degeneration in stages XIV to II (A3-to-Intermediate). The maturation and division of B type spermatogonia and maturation of preleptotene spermatocytes to the zygotene stage appeared to be independent of hormones. Maturation of pachytene spermatocytes was hormone dependent, and TP completely supported their development, meiotic division and spermiogenesis in the complete absence of pituitary hormones.
采用精子发生定量分析方法，研究了斯普拉格-道利大鼠睾丸生殖细胞对激素的依赖性。对成年去垂体大鼠每日注射丙酸睾酮（TP），持续30天。完整和去垂体的对照大鼠仅给予赋形剂。利用适合在睾丸高碘酸-希夫氏-苏木精染色切片上进行形态学鉴定的既定标准，将精原细胞分为未分化型或分化型。细胞计数数据表明，未分化精原细胞可能部分依赖于TP和/或垂体激素。分化型精原细胞组依赖于垂体激素，TP仅通过部分保护它们在第十四至第二阶段（A3至中间型）免于自发退化，部分恢复其数量。B型精原细胞的成熟和分裂以及前细线期精母细胞至偶线期的成熟似乎与激素无关。粗线期精母细胞的成熟依赖于激素，在完全缺乏垂体激素的情况下，TP完全支持其发育、减数分裂和精子发生。
