Svensson J, Eneroth P, Gustafsson J A, Ritzén M, Stenberg A
J Endocrinol. 1979 Sep;82(3):395-401. doi: 10.1677/joe.0.0820395.
The reduction of 4-[1,2-3H]androstene-3,17-dione (androstenedione) in vitro by scrotal skin was measured in samples from nine men (16--34 years old) with hypospadias and from ten male control subjects. The reduction of androstenedione was also studied in axillary and upper arm skin of seven control subjects. Androstenedione was reduced to material with chromatographic characteristics of 5 alpha-androstane-3,17-dione and to 3 alpha- and 3 beta-hydroxy-5 alpha-androstan-17-one. No difference in 5 alpha-reductase activity (defined as the sum of these three metabolites formed) was found in scrotal skin from hypospadic and control men. The mean concentration of 5 alpha-dihydrotestosterone in serum from men with hypospadias was lower than that in serum from control subjects (P less than 0.01). The mean ratio of the serum concentrations of testosterone and 5 alpha-dihydrotestosterone was higher in hypospadic men than in control subjects (P less than 0.05). No differences between the two groups were found in the mean serum concentrations of LH, FSH, prolactin, dehydroepiandrosterone, androstenedione, testosterone or testosterone-binding globulin.
在9名患有尿道下裂的男性（16 - 34岁）和10名男性对照受试者的样本中，测量了阴囊皮肤对4 - [1,2 - ³H]雄烯二酮（雄烯二酮）的体外还原作用。还在7名对照受试者的腋窝和上臂皮肤中研究了雄烯二酮的还原作用。雄烯二酮被还原为具有5α - 雄烷 - 3,17 - 二酮色谱特征的物质以及3α - 和3β - 羟基 - 5α - 雄甾 - 17 - 酮。在患有尿道下裂的男性和对照男性的阴囊皮肤中，未发现5α - 还原酶活性（定义为这三种代谢产物形成的总和）有差异。患有尿道下裂的男性血清中5α - 二氢睾酮的平均浓度低于对照受试者血清中的浓度（P < 0.01）。尿道下裂男性血清中睾酮与5α - 二氢睾酮的平均浓度比值高于对照受试者（P < 0.05）。两组在促黄体生成素（LH）、促卵泡生成素（FSH）、催乳素、脱氢表雄酮、雄烯二酮、睾酮或睾酮结合球蛋白的平均血清浓度方面未发现差异。
