虹鳟鱼离体膀胱中钠和氯的协同转运机制。
The mechanism of coupled transport of sodium and chloride in isolated urinary bladder of the trout.
作者信息
Fossat B, Lahlou B
出版信息
J Physiol. 1979 Sep;294:211-22. doi: 10.1113/jphysiol.1979.sp012926.
Abstract
- Transepithelial fluxes of Na and Cl were measured in dissected trout bladders mounted in Ussing chambers. The mucosa-to-serosa fluxes appeared to comprise a linear and a saturating (Km = 8 mM) component as a function of the luminal concentration of the ion. The over-all permeability for Cl was higher than for Na. Removal of either ion from the mucosal side induced the disappearance of the net flux of the co-ion even when the substituting ions were not impermeant. This indicated a complete and specific mutual dependence between the Na and Cl transports. A large part (30-50%) of Cl exchanges was ascribed to an exchange-diffusion process. 2. In order to localize the site of ion copuling, the apical uptakes of Na and Cl were measured by short-term exposure (2 min) to radioisotopes. The apical fluxes were similar to the transepithelial fluxes and therefore appeared to be the limiting factor for ion transfer. Ionic replacements on the mucosal side showed that the coupling between Na and Cl and Cl/Cl exchange occurs at this step.
摘要
- 在置于尤斯灌流小室中的离体虹鳟膀胱中测量了钠和氯的跨上皮通量。黏膜到浆膜的通量似乎由一个线性成分和一个饱和成分（Km = 8 mM）组成，该饱和成分是离子腔内浓度的函数。氯的总体通透性高于钠。即使替代离子并非不可渗透，从黏膜侧去除任何一种离子都会导致共离子净通量消失。这表明钠和氯的转运之间存在完全且特定的相互依赖性。很大一部分（30 - 50%）的氯交换归因于交换扩散过程。2. 为了定位离子耦合位点，通过短期（2分钟）暴露于放射性同位素来测量钠和氯的顶端摄取量。顶端通量与跨上皮通量相似，因此似乎是离子转运的限制因素。黏膜侧的离子替代表明，钠和氯之间的耦合以及氯/氯交换在此步骤发生。
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2
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3
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