Matthias F R, Hocke G
Biochim Biophys Acta. 1976 Apr 14;427(2):569-74. doi: 10.1016/0005-2795(76)90199-9.
Separation of fibrinogen degradation products D and E by means of gel chromatography cannot be achieved at neutral pH even in the presence of high ionic strength of the elution buffer. It is assumed that fragments D and E are linked together in a complex preventing the separation despite different molecular weights of both components. By means of addition of chaotropic substances like 1 M Kl to the elution buffer clear separation of degradation products D and E on Sephadex G-200 columns can be achieved.
即使在洗脱缓冲液具有高离子强度的情况下，在中性pH值下通过凝胶色谱法也无法实现纤维蛋白原降解产物D和E的分离。据推测，片段D和E以复合物的形式连接在一起，尽管两种成分的分子量不同，但仍阻止了分离。通过向洗脱缓冲液中添加离液剂（如1M碘化钾），可以在葡聚糖凝胶G - 200柱上实现降解产物D和E的清晰分离。
