兔IgG抗体形成的免疫沉淀物对人补体系统的激活机制。

The activation mechanism of human complement system by immune precipitate formed with rabbit IgG antibody.

作者信息

Fujita T

出版信息

Microbiol Immunol. 1979;23(10):1023-31. doi: 10.1111/j.1348-0421.1979.tb00532.x.

DOI:10.1111/j.1348-0421.1979.tb00532.x

Abstract

Immune precipitate (Ippt) formed between egg albumin and rabbit IgG antibody activated both pathways of the human complement system. On incubation with diluted serum, Ippt combined with several factors in the serum to form a complex which acquired C3- and C5-cleaving activities. In serum chelated with ethyleneglycol tetraacetic acid (EGTA), C3- and C5-cleaving activities of properdin system enzymes were formed on Ippt. Kinetic studies on the formation and the decay of C3- and C5-cleaving enzymes on Ippt revealed that C3- and C5-cleaving activites were almost dependent on the properdin system enzymes. The experiments in which C3-cleaving activity formed on Ippt was inhibited by anti-properdin or anti-B but not by anti-C4 supported the above results. The participation of the classical pathway was considered to accelerate the assembly of the properdin system enzymes.

摘要

卵清蛋白与兔IgG抗体之间形成的免疫沉淀物（Ippt）激活了人类补体系统的两条途径。与稀释血清孵育时，Ippt与血清中的几种因子结合形成复合物，该复合物获得了C3和C5裂解活性。在与乙二醇四乙酸（EGTA）螯合的血清中，Ippt上形成了备解素系统酶的C3和C5裂解活性。对Ippt上C3和C5裂解酶的形成和衰变进行的动力学研究表明，C3和C5裂解活性几乎依赖于备解素系统酶。用抗备解素或抗B抑制Ippt上形成的C3裂解活性，但抗C4不能抑制，上述实验支持了上述结果。经典途径的参与被认为加速了备解素系统酶的组装。