Pekkanen T J
Acta Vet Scand. 1971;12(4):523-35. doi: 10.1186/BF03547721.
Seven healthy male cats weighing between 2.35 and 4.30 kg were daily fed a diet which contained Hg labelled methyl mercury hydroxide in liver homogenate. Eight additional cats were kept as controls on a similar diet without methyl mercury hydroxide. The daily amount of methyl mercury hydroxide fed to the cats, expressed as inorganic mercury, varied between 3.75 and 4.33 mg per cat. When the animals had developed neurological symptoms typical of methyl mercury poisoning, they were decapitated and their livers removed for the determination of the mercury content, the distribution of acid phosphatase during autolysis at 37°G, the pH and the total bacterial count before and after a 24 hr. period of autolysis. Similar determinations except for the mercury were made from the livers of the control animals. The total amount of methyl mercury hydroxide fed to the cats varied between 29.14 and 40.12 mg Hg++ per kg of body weight, and the mercury content in their livers between 102.5 and 128.7 mg Hg++ per kg of liver wet weight. The share of un sedimentable activity of acid phosphatase out of the total immediately after decapitation was found to be significantly (P < 0.001) greater in the livers of the methyl mercury-fed cats than in the livers of the control animals. After 12 to 24 hrs. of autolysis at 37 °G the unsedimentable activity accounted for 100 % of the total acid phosphatase activity in the livers of 6 of the 7 methyl mercury-fed animals, while in the livers of the controls the corresponding percentages varied after 24 hrs. of incubation between 42 and 73, the mean being 57.4 ± 11.4 %. The mean pH of the livers of the methyl mercury fed animals was found to be significantly higher before (P< 0.001) and after (P < 0.001) a 24 hr. incubation at 37 °G than the corresponding mean pH values of the control animals. Because of the injection of antibiotics given to the cats before sacrifice the total bacterial count of the livers, which was checked before and after a 24 hr. incubation at 37°G, was found to be zero.
7只体重在2.35至4.30千克之间的健康雄性猫，每天喂食含有肝脏匀浆中汞标记的氢氧化甲基汞的日粮。另外8只猫作为对照，喂食不含氢氧化甲基汞的类似日粮。每只猫每天喂食的氢氧化甲基汞量，以无机汞计，在每只猫3.75至4.33毫克之间。当动物出现典型的甲基汞中毒神经症状时，将其断头并取出肝脏，以测定汞含量、37℃自溶过程中酸性磷酸酶的分布、pH值以及自溶24小时前后的细菌总数。对照动物的肝脏除汞含量外进行类似测定。喂给猫的氢氧化甲基汞总量在每千克体重29.14至40.12毫克汞离子之间，其肝脏中的汞含量在每千克肝脏湿重102.5至128.7毫克汞离子之间。发现断头后立即测定，喂食甲基汞的猫肝脏中酸性磷酸酶不可沉降活性占总活性的比例显著高于对照动物肝脏（P<0.001）。在37℃自溶12至24小时后，7只喂食甲基汞的动物中有6只肝脏中不可沉降活性占酸性磷酸酶总活性的100%，而对照动物肝脏在孵育24小时后相应比例在42%至73%之间变化，平均值为57.4±11.4%。发现喂食甲基汞的动物肝脏在37℃孵育24小时前（P<0.001）和后（P<0.001）的平均pH值显著高于对照动物相应的平均pH值。由于在处死前给猫注射了抗生素，经37℃孵育24小时前后检查，发现肝脏的细菌总数为零。
