胰岛素对大鼠骨骼肌体外游离氨基酸池及蛋白质合成的影响。
The effect of insulin on free amino acid pools and protein synthesis in rat skeletal muscle in vitro.
作者信息
Hider R C, Fern E B, London D R
出版信息
Biochem J. 1971 Dec;125(3):751-6. doi: 10.1042/bj1250751.
Abstract
- The effects of insulin in vitro on tissue pools and incorporation into protein of glycine and leucine in the extensor digitorum longus muscle of the rat are reported. 2. It was found that insulin decreased the lag period before the establishment of a linear rate of incorporation of radioactive glycine into protein. 3. The hormone increased the size of the free intracellular glycine pool. No such effect was found for leucine. The accumulation of radioactive glycine in the intracellular fluid compartment was increased. The content of radioactive leucine in the intracellular compartment was decreased. 4. Insulin decreased the specific radioactivity of both glycine and leucine in the extracellular fluid. 5. The hormone also decreased protein catabolism. 6. The effect on protein synthesis was not caused by an increase in the specific radioactivity of the extracellular pool but was possibly related to increased amino acid concentrations in this pool, which could in turn have affected the aggregation of ribosomes.
摘要
- 本文报道了胰岛素在体外对大鼠趾长伸肌中甘氨酸和亮氨酸的组织池以及掺入蛋白质情况的影响。2. 研究发现，胰岛素缩短了放射性甘氨酸掺入蛋白质达到线性速率之前的延迟期。3. 该激素增加了细胞内游离甘氨酸池的大小。亮氨酸未发现此效应。细胞内液区室中放射性甘氨酸的积累增加。细胞内区室中放射性亮氨酸的含量降低。4. 胰岛素降低了细胞外液中甘氨酸和亮氨酸的比放射性。5. 该激素还降低了蛋白质分解代谢。6. 对蛋白质合成的影响并非由细胞外池比放射性的增加所致，而是可能与该池中氨基酸浓度的增加有关，这反过来可能影响了核糖体的聚集。
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